油菜外植体离体培养的细胞分化研究  

Study of Cytogenesis on Rape Explants in vitro Culture

作　　者：卢龙斗[1] 丁笑生[1] 孙富丛[1] 段红英[1] 贾美丽[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《华北农学报》2005年第2期23-25,共3页Acta Agriculturae Boreali-Sinica

基　　金：河南省自然科学基金项目(0124160310)

摘　　要：为了探索油菜外植体分化再生的机理,利用扫描电镜技术对芥菜型油菜DB3的下胚轴、子叶在不同的离体培养时期的细胞分化和形态变化进行了研究。结果表明,下胚轴外植体是切面上的维管束薄壁细胞和距离切面400～600μm的薄壁细胞脱分化,然后再分化形成芽。而子叶仅仅是距离切面400～600μm的薄壁细胞脱分化后再分化成芽的。In this study,hypocotyl and cotyledon of B.juncea,cultured in vitro at different time,were scanned by scanning microscope.The results indicated there were two kinds of cells de-differentiated and then differentiated shoots in hypocotyls. One was parenchyma cells of hypocotyl vascular bundle on cut face,the other was parenchyma cells about 400-600 μm away from cut face. But parenchyma cells of cotyledon did only from the later.

关 键 词：细胞分化 离体培养 外植体 细胞脱分化 扫描电镜技术 芥菜型油菜 形态变化 薄壁细胞 下胚轴 再分化 DB3 600 子叶 距离 维管 

分 类 号：S682.11[农业科学—观赏园艺] Q813[农业科学—园艺学]