白细胞介素-6基因启动子多态性与血脂及血小板计数关系的研究  

Relationship Between Interleukin-6 Gene Promoter Polymorphisms and Plasma Lipid, Platelet Count

作　　者：韦叶生[1] 刘运广[1] 李壮[1] 常正义[1] 覃羽华[1] 

机构地区：[1]右江民族医学院附属医院临床检验中心,广西百色533000

出　　处：《中国医师杂志》2005年第5期583-585,共3页Journal of Chinese Physician

基　　金：广西卫生厅课题(Z2004037);广西教育厅课题(桂教科200420);广西青年科学基金资助项目(桂科青0447060)

摘　　要：目的研究白细胞介素-6(IL-6)基因启动子-572C/G、-634C/G多态性在广西地区汉族人群中的分布特点,并探讨其与血脂、血小板计数的关系。方法应用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)检测198例正常汉族人IL-6基因型,同时检测血小板数量及血脂水平。结果IL-6的基因型间血脂水平比较差异无显著性(P>0.05);IL-6基因-572C/G多态性与血小板数量具有相关性,携带G等位基因的个体血小板数量显著高于不携带者(178.75±49.32×109·L-1vs169.87±47.19×109·L-1,P<0.05),IL-6基因-634C/G多态性与血小板数量无相关性(P>0.05)。结论在广西汉族人群中存在IL-6基因-572C/G、-634C/G多态性;且IL-6基因-572C/G多态性与血小板数量具有相关性,携带G等位基因的个体血小板数量显著高于不携带者,这在血栓性疾病及动脉粥样硬化预防中具有重要意义。Objective To study the distribution of IL-6 gene promoter 572C/G, 634C/G polymorphisms in Han population of Guangxi province, and analyze the relation between IL-6 gene polymorphisms and plasma lipid, platelet count. Methods Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was used to detect IL-6 genotype in 198 healthy Han adults. At the same time the plasma lipid level and platelet count were determined by routine methods. Results Serum lipid levels had not significant difference among the different genotypes of IL-6 (P<0.05). IL-6 gene 572C/G polymorphism was associated with platelet count, and G allele carriers had higher platelet count than non-carriers (178.75±49.32×109·L^-1 vs 169.87±47.19×109·L^-1 ,P<0.05). IL-6 gene -634C/G polymorphism was not associated with platelet count. Conclusion IL-6 gene polymorphisms were found in healthy Han population of Guangxi province. IL-6 gene 572C/G polymorphism was associated with platelet count, and G allele carriers had higher platelet count than non-carriers, which may had important significance for preventing thrombotic diseases and atherosclerosis.

关 键 词：血小板计数 基因启动子多态性 白细胞介素-6 聚合酶链反应-限制性片段长度多态性法 IL-6基因 血小板数量 动脉粥样硬化 汉族人群 血脂水平 等位基因 人IL-6 血栓性疾病 相关性 广西地区 方法应用 同时检测 重要意义 基因型 

分 类 号：R446.1[医药卫生—诊断学]