支气管哮喘气道IL_4和IL_(12)动态变化及对气道炎症反应的影响  

The Effect of IL-4, 12 on the Airway Inflammation in Rat Asthma

作　　者：欧立文[1] 张平[1] 杨祚升[1] 龙治峰[1] 贺兼斌[1] 王小华[1] 

机构地区：[1]南华大学第一附属医院呼吸内科,湖南衡阳421001

出　　处：《中国医师杂志》2005年第5期626-628,共3页Journal of Chinese Physician

基　　金：湖南省教育厅科研资助项目(O3C419)

摘　　要：目的研究支气管哮喘(哮喘)炎症促进因子白细胞介素4(IL-4)和炎症抑制因子(IL-12)的变化趋势,相互关系及对气道炎症反应的影响。方法用卵蛋白(OVA)敏原制备wistar大鼠哮喘模型,在激发后1、8、24、48、96h及7、14d行支气管肺泡灌洗(BAL),测定支气管肺泡灌洗液(BALF)中炎症细胞总数与嗜酸细胞数(EOS);酶联免疫吸附法(ELISA)测定BALF中IL-4、IL-12的浓度。结果OVA激发后,气道出现明显的炎症反应。EOS计数1h上升,8h后迅速上升,24h达到高峰期,96h后开始回落,7～14d回落到略高于对照组;炎症细胞总数1h上升,8h后迅速上升,24～96h处于高峰期,7d回落到接近8h水平,14d接近基础水平。BALF中,IL-4在1h上升,8～96h处于高峰期,7～14d回落到基础水平;IL-12在1h上升,8处于低水平,24h上升,48～96h处于高峰期,7～14d回落到基础水平;EOS与IL-4有正相关性(r=0.74,P<0.001),与IL-12无相关性(r=-0.13,P>0.05),与IL-4/IL-12有密切相关性(r=0.85,P<0.001)。结论哮喘气道中存在IL-4/IL-12比值失衡,IL-4/IL-12比值对哮喘气道炎症反应起重要的调节作用。Objective To investigate the level change of interleukin-4(IL-4) and IL-12, and their effect on airway inflammation in rat asthma. Methods Rat model of asthma was set up by ovalbumin sensitization. Total cell count and eosinophil count in branchoalveolar lavage fluid(BALF) were performed, and levels of IL-4 and IL-12 in BALF were measured by ELISA. Results After ovalbumin sensitization, airway occurred obvious inflammation. Eosinophil count began increasing at 1 hour after sensitization, obviously increased at 8 hours, reached a maximum at 24 hours, started declining at 96 hours, and decreased to near normal range at 7 to 14 days. The changes of total cell count had the same tendency as eosinophil count changes. The level of IL-4 in BALF elevated at 1h after sensitization, reached a maximun at 8 to 96 hours, and reduced to near normal level at 7 to 14 days. The changes of IL-12 in BALF had the same tendency as IL-4 level changes. Eosinophil count was positively related with IL-4 level(r=0.74,P<0.001), and no significant correlation was found between IL-12 and eosinophil count(r=-0.13,P>0.05). There was a close correlation between IL-4/IL-12 ratio and eosinophil count (r=0.85,P<0.001). Conclusion There was unbalance of IL-4/IL-12 ratio in BALF of rat asthma, and IL-4/IL-12 ratio might play an important role in branchoalveolar inflammatory reaction.

关 键 词：气道炎症反应 支气管哮喘 动态变化 IL12 支气管肺泡灌洗(BAL) IL4 IL-12 Wistar大鼠 支气管肺泡灌洗液 酶联免疫吸附法 IL-4 基础水平 细胞总数 BALF 白细胞介素 嗜酸细胞数 高峰期 相关性 抑制因子 促进因子 相互关系 

分 类 号：R562.25[医药卫生—呼吸系统] R743.33[医药卫生—内科学]