化纤方对人胚肺二倍体纤维母细胞的增殖抑制作用  

Effect of Huaxianfang on the Proliferation of 2BS Cells

作　　者：闫智勇[1] 张天娥[2] 刘明芳[1] 罗全安[1] 李文军[2] 李戎[2] 蔡永宁[1] 李志刚[3] 

机构地区：[1]四川大学华西医学中心,四川成都610041 [2]成都中医药大学,四川成都610075 [3]成都理工大学,四川成都610059

出　　处：《华西医学》2005年第2期280-281,共2页West China Medical Journal

基　　金：四川省科技厅重点攻关项目(03SG011-013)

摘　　要：目的:探讨中药化纤方对人胚肺二倍体纤维母细胞(2BS)增殖的抑制作用及其机理,为中医药介入肺纤维化防治提供实验和理论支持。方法:采用中药血清药理学实验方法,观察化纤方含药血清对2BS细胞的细胞增殖、细胞凋亡和细胞周期的影响。结果:经化纤方含药血清作用后,2BS细胞增殖受到显著抑制,并出现明显的细胞凋亡现象;化纤方各组S期细胞数显著增加,而G2/M期细胞数则明显下降,提示该方药可阻抑S期细胞向G2/M期的移行,从而抑制其分裂增殖。结论:化纤方有明显的抗肺纤维化作用,其作用机制可能是阻抑细胞增殖周期并诱导其发生细胞凋亡。Objective:To investigate the effects and mechanism of the Chinese Compound Prescription Huaxianfang(HXF)on proliferation of 2BS cells.Methods:By using the method of pharmacology of the serum with Chinese drugs(PSCD),the HXF drug-serum was incubated with cultured 2BS cells.The cell proliferation and apoptosis were observed.Results:After treating with the drug-serum,the proliferation of 2BS cells was inhibited evidently.The cells showed characters of apoptosis.Compared with the control,the cell percent of S phase was increased and that of G 2/M phase were decreased.Conclusion:HXF cloud significantly inhibit the proliferation of 2BS cells and promote the apoptosis of these cells,which may be the important mechanisms of HXF' treatment of pulmonary fibrosis.

关 键 词：化纤方 人胚肺 二倍体纤维母细胞2BS 中药 药理学 细胞增殖 细胞抑制 细胞凋亡 肺间质纤维化 

分 类 号：R285[医药卫生—中药学]