高效液相色谱法测定鼠脑组织中氨甲喋呤浓度  

Determination of MTX in Brain Tissue by High-Performance Liquid Chromotograph

作　　者：高山[1] 游潮[2] 樊均明[1] 苑树立 王德明[1] 张晓强[1] 

机构地区：[1]广安市人民医院神经外科,四川广安638001 [2]四川大学华西医院神经外科,四川成都610041

出　　处：《华西医学》2005年第2期288-289,共2页West China Medical Journal

摘　　要：目的:建立高效液相色谱法(HPLC法)测定鼠脑组织中氨甲喋呤(MTX)浓度的方法。方法:脑组织样加入高氯酸,残渣经流动相溶解后测定,检测波长为306nm。结果:相关系数r=0.9997;线性范围:10.0～0.078125μg.ml-1;最低定量浓度为0.078125μg.ml-1;日内RSD≤7.00%,日间RSD≤6.44%,萃取回收率RSD为3.28%～5.48%。结论:本方法简便、可靠,灵敏度高,可用于氨甲喋呤在大鼠脑组织内的检测和药代动力学研究。Objective:To establish the valid detection method with the HPLC system for the determination of concentration of MTX in rat brain.Methods:MTX was extracted from brain tissue get in with HClO 4 and soluted by a mobile phase and then detected at 306nm. Results:The correlation coefficients was 0.9997.The calibration curve was linear over the range of 10.0～0.078μg.ml -1,and the limit of detection was 0.078μg.ml -1.The average relative deviations of within day and between day variation were 2.82%～7.00% and 4.19～6.44% respectively.The average relative deviations of recovery of MTX in sample was 3.28%～5.48%.Conclutions:The method was simple,sensitive and accrate.It was shown to be suitable for monitoring and pharmacokinetic studies of MTX in rat brain.

关 键 词：高效液相色谱法 氨甲喋呤 脑组织浓度 抗代谢类 抗肿瘤药物 

分 类 号：R965[医药卫生—药理学]