成年大鼠心肌细胞的分离和培养  

Isolation and Culture of Adult Rat Ventricular Cardiomyocytes

作　　者：李勇刚[1] 石应康[1] 陈焕文[1] 刘小菁[2] 丁怡[3] 王树人[3] 

机构地区：[1]四川大学华西医院胸心外科 [2]四川大学华西医院心血管疾病研究室 [3]四川大学基础医学院病理生理教研室,四川成都610041

出　　处：《华西医学》2005年第2期308-309,共2页West China Medical Journal

基　　金：国家自然科学基金(批准号:30270567)资助

摘　　要：目的:建立获得较高比例的活性成年大鼠心肌细胞的分离和培养方法。方法:Langendorff灌流,胶原酶消化分离钙耐受成年大鼠心肌细胞,加血清培养。光镜观察,结合形态学和台盼蓝染色评价心肌细胞。结果:分离的活性心肌细胞比率约70%～80%,杆状、横纹清晰。加血清贴壁培养的细胞逐渐发生形态学改变。结论:通过本方法可以对成年大鼠心肌细胞进行良好的分离培养。Objective:To build a useful method of isolation and culture of adult rat ventricular cardiomyocytes.Methods:Calcium tolerant cardiomyocytes were isolated with collagenase by Langendorff perfusion and cultured in serum-supplemented Medium 199.The cardiomyocytes were morphologically observed with optical microscope and evaluated through trypan blue dying.Results:Viability of freshly isolated adult rat ventricular cardiomyocytes,which were rod-shaped with clear cross-striations,was 60%～80%.The cultured cardiomyocytes attached to the culture plate bottom and gradually became rounded.Conclusion:Adult rat ventricular cardiomyocytes can be isolated and cultured well with the above method.

关 键 词：大鼠 心肌细胞 细胞分离 细胞培养 心肌结构 

分 类 号：R541[医药卫生—心血管疾病]