5个山羊品种CSN1S2基因的Alw26Ⅰ酶切多态性分析  

Polymorphism Analysis of the CSN1S2 Gene Digested with Alw26Ⅰ in Five Goat Breeds

作　　者：蓝贤勇[1] 陈宏[1] 张润锋[1] 田燚[1] 雷初朝[1] 潘传英[1] 罗军[1] 胡沈荣[1] 

机构地区：[1]西北农林科技大学动物科技学院

出　　处：《遗传》2005年第3期363-366,共4页Hereditas（Beijing)

基　　金：农业部留学人员活动基金(编号:199912D);陕西省自然科学基金(编号:99SM06)~~

摘　　要：利用PCR RFLP技术对西农萨能奶山羊、关中奶山羊、陕南白山羊、安哥拉山羊和波尔山羊5个品种的170个个体的αs2酪蛋白(CSN1S2)基因进行多态性分析,结果表明:扩增大小为310bp的片段经限制性内切酶Alw26Ⅰ酶切后表现多态,且5个山羊品种该基因座位均处于Hardy Weinberg平衡状态。西农萨能奶山羊、关中奶山羊、陕南白山羊、安哥拉山羊和波尔山羊的基因杂合度/有效等位基因数/Shaanon信息熵/PIC值分别为0.1589/1.1889/0.2955/0.1463,0.4114/1.6981/0.6017/0.5171,0.1653/1.1980/0.3046/0.1516,0.0646/1.0691/0.1463/0.0625,0.0541/1.0572/0.1270/0.0526。分析结果显示,关中奶山羊的遗传多样性最丰富,表现为高度多态;其次是西农萨能奶山羊和陕南白山羊,而安哥拉山羊和波尔山羊的遗传变异程度最低。PCR-RFLP was applied to analyze the polymorphism of CSN1S2 gene in 170 goats that comprised of five goat breeds, namely Xinong Saanen dairy goat, Guanzhong dairy goat, Shaannan white goat, Angora goat and Boer goat. A 310 bp -long PCR product was digested with Alw26Ⅰ and demonstrated polymorphism in five goat populations that were all at Hardy-Weinberg equilibrium (P>0.05). For Xinong Saanen dairy goat, Guanzhong dairy goat, Shaannan white goat, Angora goat and Boer goat, gene heterozygosity/effective allele gene number/Shaanon information entropy/polymorphism information content were 0.1589/1.1889/0.2955/0.1463, 0.4114/1.6981/0.6017/0.5171, 0.1653/1.1980/0.3046/0.1516, 0.0646/1.0691/0.1463/ 0.0625, 0.0541/1.0572/0.1270/ 0.0526, respectively. According to the genetic diversity indexes described above of the five goat breeds, Guanzhong dairy goat had the most abundant genetic diversity and showed high polymorphism, and Xinong Saanen dairy goat and Shaannan white goat were inferior, while Angora goat and Boer goat had the lowest genetic variability.

关 键 词：山羊 CSN1S2基因 PCR-RFLP 多态性 

分 类 号：S827[农业科学—畜牧学]