鸡种系嵌和体的研制及其AFLP检测  

Generation of Chicken Germ-line Chimeras by Transferring PGCs and Their Identification by AFLP

作　　者：胡小芬[1] 谢蓓[1] 于瑞嵩[1,2] 黄启忠[1] 张德福[1,2] 黄路生[3] 李震[1,2] 

机构地区：[1]上海市农业科学院畜牧兽医研究所 [2]上海市农业遗传育种重点实验室,上海201106 [3]江西农业大学江西省动物生物技术重点开放实验室

出　　处：《遗传》2005年第3期367-371,共5页Hereditas（Beijing)

基　　金：上海市农业科学院中青年学术技术带头人培养基金资助~~

摘　　要：利用密度梯度离心等方法从孵化51～56h的石歧杂鸡胚血液中提取PGCs,用自制的玻璃注射针将PGCs注射到孵化2.5d的H系受体鸡胚中制备种系嵌和体鸡;通过筛选AFLP引物建立起家禽嵌和体的AFLP检测方法;经检测20个发育的PGCs受体鸡胚中有8个种系嵌和体,嵌和率为40%。PGCs (Primordial germ cells) were isolated from the blood of 51~56 h hatching Shiqiza chicken embryos by Ficoll density gradient centrifugation. The PGCs were injected into 2.5 d hatching embryos of H breed chicken to produce germ-line chimeras. AFLP checking method was established to identify chicken germline chimeras. Eight germ-line H-S chimera embryos were identified among 20 developing H breed embryos.

关 键 词：家禽原始生殖细胞 种系嵌和体 扩增片段长度多态性 

分 类 号：S814.8[农业科学—畜牧学]