RTA-RA_3是B_(220)特异性的有潜力的抗自身免疫的免疫毒素  

IMMUNOTOXIN RTA-RA_3 WITH ANTI-B_(220)ACTIVATY,A USEFUL AGENT IN TREATMENT OF AUTOIMMUNE DISEASES

作　　者：吴敏[1] 林美淳[1] 张育华[1] 曾云[1] 周维其 陈正芳[1] 任德莲[1] 能势真人 

机构地区：[1]泸州医学院微生物学教研室,646000 [2]日本东北大学病理学部

出　　处：《泸州医学院学报》1994年第1期1-5,共5页Journal of Luzhou Medical College

基　　金：国家自然科学基金!39170701;卫生部青年科学基金;四川省科委基金

摘　　要：将抗B220单克隆抗体分泌克隆RA3注射至SCID小鼠腹腔以获取腹水，抗体经proteinA亲和层析柱提纯，再经SPDP双功能交联剂修饰后，与还原的去糖基蓖麻毒蛋白A链反应生成免疫毒素RTA-RA3．SephadexG150柱层析分离纯化此免疫毒素，在还原状态下可见到免疫球蛋白重链、轻链及另一条带RTA．免疫毒素体内注射，研究MRL/＋和MRL/lpr小鼠脾细胞的淋巴细胞标志．实验表明：RTA－RA3对脾B220＋细胞有显著抑制作用．提示这一免疫毒素可用于治疗自身免疫性疾病。The monoclonal antibody was obtained by injecting RA3 hybridoma cells into the abdominal cavity to develop the ascites and then purified by protein A sepharose 4B affinity column. The antibody then was subjected to modification by SPDP bifunctional reagent,and conjugated with the deglycosylated ricin A chain by reducing the latter molecule. The coupled immunotoxin RTA-RA3 was separated from the free antibody and free RTA by Sephadex G-150 column. In the SDS-PAGE gel, the reduced immunotoxin appeared in three bands, 50K, 25K, and 30K, Corresponding to immunoglobulin heavy chain, light chain, and ricin A chain respectively. In vivo injection of the immunotoxin into the MRL/lpr and MRL/- .mice was done through tail vein, after 2 days the splenocytes were harvested and surface markers of the cells were investigated by flow cytometry. The results show that the immunotoxin RTA-RA3 can selectively clear the B220+ splenocytes, therefore, the new anti-B220+ immunotoxin is very promising in treatment of the autoimmune diseases.Further research is under progress.

关 键 词：蓖麻毒蛋白A链 自身免疫 单克隆抗体 

分 类 号：R392.11[医药卫生—免疫学]