应激心肌损伤的蛋白质组学研究  

PROTEOMICS ANALYSIS ON STRESSED MYOCARDIUM INJURY-RELATED PROTEINS

作　　者：弓景波[1] 吴淑庆[1] 钱令嘉[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《中国应用生理学杂志》2005年第2期171-174,共4页Chinese Journal of Applied Physiology

基　　金：国家重点基础研究发展项目资助课题(G20000569);国家自然科学基金资助课题(30070283)

摘　　要：目的:寻找应激心肌损伤相关蛋白。方法:建立束缚应激心肌损伤模型,制备心室肌2DE蛋白样品和心肌2DE图谱,图像分析软件分析应激后蛋白表达差异点,MALDI TOF MS数据库搜索鉴定蛋白质。结果:应激前后10个蛋白表达量发生改变,其中8个应激后表达显著升高,经质谱鉴定为心肌肌球蛋白、白蛋白、脂蛋白A I前体等;2个显著降低,经质谱鉴定为线粒体能量代谢酶类和UCP3。结论:这些差异蛋白可能参与应激机体心肌损伤的发生。Aim: To probe the related proteins to stress-induced myocardium injury. Methods: After establishment of a myocardium injury model induced by restraint stress in rats, myocardium proteins of restraint stress-treated and untreated rats were extracted, and the two-dimensional polyacrylamide gel electrophoresis (2-DE) maps of the extracted proteins were established by using the immobilized pH gradient (IPG) and SDS-PAGE two-dimensional electrophoresis respectively. The alterative protein spots were analyzed by (Image) Master 3.01 software and identified with assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching. Results: Proteomics analysis showed that there were 10 proteins were significantly influenced by restraint stress in rat myocardium. After stress, proteins, including cardiac myosin heavy chain, dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex, similar to dihydrolipoamide S-succinyltransferase, mitochondrial aldehyde dehydrogenase, H(+)-transporting ATP synthase, albumin, myosin heavy chain and apolipoprotein A-I precursor showed increased expression. Mitochondrial aconitase and uncoupling protein UCP-3 showed decreased expression. Conclusion: These differential expressive proteins might be involved in stress-induced injury to myocardium.

关 键 词：应激 心肌损伤 蛋白质组学 

分 类 号：Q17[生物学—水生生物学]