免疫卵黄特异性阻断家蚕浓核病感染的研究  

Study on Specific Yolk Antibody to Prevent DNV From Infecting Silkworm ,Bombyx mori

作　　者：杨琼[1] 廖森泰[1] 方定坚[1] 郑祥明[1] 肖更生[1] 罗国庆[1] 吴福泉[1] 唐翠明[1] 黄炳辉[1] 林克义[2] 梁眷蘅[2] 尹烨华[2] 

机构地区：[1]广东省农业科学院蚕业研究所,广州510610 [2]广东省农业科学院兽医研究所,广州510640

出　　处：《蚕业科学》2005年第2期224-227,共4页ACTA SERICOLOGICA SINICA

基　　金："十五"国家重点科技攻关子专题(编号2001BA502B010203;广东省科技计划项目(编号2003C33504)

摘　　要：用家蚕浓核病病原(Bombyxmoridensonucleosisvirus,BmDNV)免疫蛋鸡收集的卵黄,通过体外中和与体内中和生物试验测定鸡免疫卵黄对DNV的防治效果。结果表明,免疫的鸡卵黄能特异性阻断DNV感染家蚕,达到预防的目的,而非免疫卵黄未表现有阻断作用。结果提示:鸡免疫卵黄中含有抗DNV的有效抗体。Yolk immunoglobulings(IgY)were achieved by immunizing about 20 weeks hens using silkworm DNV as antiagen.External neutralization and internal experiments had been done to study the protective effect of IgY to DNV. The results showed IgY could reduced the infection rate by preventing DNV to infect silkworm midgut ,but non-immume normal egg yolk didn't show the prevention effect. The result infers that the immune egg yolk owned the special antibody against DNV.

关 键 词：家蚕 浓核病 免疫卵黄 

分 类 号：S884.5[农业科学—特种经济动物饲养]