TCRCα-560C/T多态性与IgA肾病临床病理的关联分析  

Association of TCRCα-560 C/T polymorphism with histological and clinical manifestations in IgA nephropathy patients of Han nationality in Chinese population

作　　者：薛超[1] 李幼姬[1] 李彩霞[2] 王一鸣[3] 胡彬[3] 陈路明[3] 黎嘉能[4] Patrick H Maxwell 

机构地区：[1]中山大学附属第一医院肾内科 [2]中山大学中山医学院医学统计学教研室,广东广州510080 [3]中山大学中山医学院医学遗传学教研室,广东广州510080 [4]香港大学玛丽医院内科 [5]英国皇家学院霍尔姆斯医院内科

出　　处：《第四军医大学学报》2005年第10期897-900,共4页Journal of the Fourth Military Medical University

基　　金：美国中华医学基金(98 677);国家自然科学基金(30170434);广东省自然科学基金(20013140)

摘　　要：目的: 探讨T细胞受体保守域Alpha链基因(TCRCα)- 560C/T多态性与我国汉族人群IgA肾病临床病理的相关关系.方法: PCR RFLP法和PCR产物直接测序法鉴定400例IgA肾病患者的基因型,按基因型分为TT(135人),TC(196人)和CC(69人)3组.与其临床病理资料进行关联分析,并追踪随访.结果: ①TCRCα560的TT基因型的患者其蛋白尿发生率显著降低(P<0. 05),而CT基因型的患者蛋白尿发生率则显著增高(P<0 01 ),CC基因型的患者发生蛋白尿的比例亦有增高趋势;②在IgA肾病病理中度病变组,CC基因型的分布频率相对于其他基因型有显著增高(P<0 .05 );③211例随访患者,TCRCα- 560C/T基因在肾功稳定组与进展组间,不论是基因型还是等位基因的分布频率均未见显著性差异(P>0 .05).结论: 中国汉族人群中,TCRCα基因-560C/T基因多态性可能和IgA肾病蛋白尿的发生及肾病病理有一定关联性,但可能与肾功能进展不相关.AIM: To investigate the relationship of TCRCα-560 C/T polymorphism with histological and clinical manifestations of IgA nephropathy patients of Han nationality in Chinese population.METHODS: TCRCα-560 C/T genotypes in 400 cases of IgA nephropathy were determined by PCR-RFLP and direct sequencing.All the cases were divided into three groups by their genotypes: TT/CT/CC=135/196/69.Clinical and pathological records of the patients were studied and the patients were followed up.RESULTS: ① The occurrence of albuminuria was found to be significantly low in TT (P<0.05) but significantly higher in CT (P<0.01) and increasing tendency was found in CC.No significant differences of genotype distribution were found in gender,age,blood pressure,urinary protein level and serum IgA level (P>0.05).② Significant differences of genotypes distribution were found in cases with moderate renal histopathology.CC genotype frequency was significantly higher than other types (P<0.05).③ Of the 211 cases which had been followed-up nearly three years,no significant distribution difference of genotypes or alleles was found between the stable group and the progressive group (P>0.05). CONCLUSION: TCRCα-560 C/T polymorphism may be associated with the occurrence of albuminuria and such renal tissue lesion as renal messangial proliferation,but may not be very helpful in predicting the progression of chronic renal failure in IgA nephropathy patients of Han nationality in Chinese population.

关 键 词：肾小球肾炎 IgA T淋巴细胞 受体 抗原 多态现象(遗传学) 

分 类 号：R692.31[医药卫生—泌尿科学]