Ⅰ、Ⅲ型前胶原核酶治疗裸鼠增生性瘢痕后胶原形态的改变  

Morphological Changes of Collagen in Hypertrophic Scar Treated with Ribozymes of Type Ⅰand Ⅲ Procollagen

作　　者：张涛[1,2] 朱家源[1] 朱斌[1] 李新强[1] 唐冰[1] 

机构地区：[1]中山大学第一附属医院烧伤科 [2]广州市红十字会医院烧伤整形科,广东广州510220

出　　处：《广州医学院学报》2005年第1期27-29,共3页Academic Journal of Guangzhou Medical College

基　　金：国家自然科学基金资助项目(No. 39770842)

摘　　要：目的:研究Ⅰ、Ⅲ型前胶原核酶治疗裸鼠增生性瘢痕后胶原含量及分布的改变。方法:①建立裸鼠增生性瘢痕动物模型(80只)。②将裸鼠随机分为:实验组(A组20只,注射Ⅰ型前胶原核酶;B组20只,注射Ⅲ型前胶原核酶;C组20只,注射Ⅰ型及Ⅲ型前胶原核酶;各组又分为2. 5μg/100μL及5. 0μg/μL两个剂量组,每个剂量组各10只裸鼠,每天用微量注射器行瘢痕内多位点注射1次,共7d)及对照组(D组10只,注射生理盐水7d;E组10只,空白对照组)。③应用苦味酸天狼猩红染色偏振光法,结合图像分析技术,观察分析各组胶原含量及分布的改变。结果:A组Ⅰ型胶原由紧密排列,变成较稀疏,提示含量减少;B组Ⅲ型胶原在血管旁及Ⅰ型胶原间分布减少;C组Ⅰ、Ⅲ型胶原均减少;D组和E组无明显变化。结论:Ⅰ、Ⅲ型前胶原核酶治疗裸鼠增生性瘢痕后,可使胶原的排列分布稀疏,含量减少,形态改变明显。Objective:To study the morphological changes of collagen in human hypertrophic scar in a nude mice model treated with ribozymes. Methods:To establish a model of human hypertrophic scar in nude mice(80 mice).All the mice were randomly divided into four groups:group A(typeⅠprocollagen ribozyme treated,20 mice), group B(type Ⅲ procollagen ribozyme treated,20 mice),group C(typeⅠand Ⅲ procollagen ribozyme treated,20 mice),group D(normal saline solution injected,10 mice)and group E(blank control,10 mice). Tow subgroups were established by microinjection of 2.5 and 5.0μg/100 μL of ribozymes of type Ⅰ and Ⅲ procollagen in groups A,B and C for 7 days. The sirius red staining and polarization microscopy, combined with image analysis were applied to observe the results. Results:The ribozymes can inhibit type Ⅰand Ⅲ collagen of hypertrophic scar. Under the polarization microscopy, the structure of scar became loose, the content and distribution of collagenous fibre diminished. There were no remarkable changes in group D and group E. Conclusions:The results suggested that ribozymes of type Ⅰand Ⅲ procollagen can inhibit collagen production, which was proved morphologically.

关 键 词：核酶 增生性瘢痕 天狼猩红 胶原 

分 类 号：R619.6[医药卫生—外科学]