核苷酸序列比对在生物系统发育研究中的应用  

ALIGNMENT OF NUCLEIC ACIDS SEQUENCES IN THE STUDY OF PHYLOGENY

作　　者：谢强[1] 卜文俊[1] 

机构地区：[1]南开大学生命科学学院昆虫学研究所昆虫分子系统学实验室,天津300071

出　　处：《动物分类学报》2005年第2期281-286,共6页Acta Zootaxonomica Sinica

基　　金：国家自然科学基金 (30 2 70 1 67);国家基础科学人才培养特殊学科点项目 (昆虫分类学 2 0 0 2 2 0 0 6)资助

摘　　要：作为确定分子数据初级同源性(primaryhomology)的过程,比对的重要性在于后续算法的选择。本文对其在生物系统发育研究中的作用及其基本步骤与算法做了简要说明;从理论与实践相结合的角度介绍了使用最为广泛的软件,讨论了其中的空位(gap)问题、参数问题和比对结果校正等问题;并且对此领域中前沿的基因组比对研究进展做了概述。The study of DNA sequence alignment originated from the seventies of the 20 th century and its application in the phylogeny developed rapidly during the spring of molecular systematics in the nineties of the 20 th century. As the process in which the primary homology of macromolecular sequences is determined, its effect on phylogenetic results is still heavier than the methods of phylogenetic reconstruction. In this paper, the basic steps and algorithm of alignment were introduced briefly, the problems of parameters, gaps and correction to the alignment results were discussed. We also summarized the progress of the alignment of genome.

关 键 词：发育研究 生物系统 序列比对 核苷酸 应用 理论与实践 分子数据 基本步骤 研究进展 同源性 基因组 算法 

分 类 号：Q75[生物学—分子生物学]