益气健脾、益气健脾祛痰化瘀方药对脑老化小鼠神经元保护作用的比较研究  

Comparative Study on Effect of Receipe for Replenishing Spleen Qi and Replenishing Spleen Qi Added with Dispelling Phlegm and Stasis on Brain-aging Mouse Neuron Protecting

作　　者：詹向红[1] 杨靖[2] 赵君玫[1] 

机构地区：[1]河南中医学院,河南郑州450008 [2]长春中医学院,吉林长春130117

出　　处：《中国中医基础医学杂志》2005年第5期338-340,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：河南省科技攻关资助项目(0324410042)

摘　　要：目的比较益气健脾、益气健脾祛痰化瘀方药对脑老化小鼠神经元保护作用。方法采用D半乳糖(D galactose,D gal)复制亚急性衰老及脑老化动物模型。以流式细胞术(flowcytometry,FCM)检测大脑神经元线粒体膜电位(Mitochondrialmembranepotential,MMP)及凋亡百分率,病理图文分析系统检测大脑神经元密度。结果益气健脾和益气健脾祛痰化瘀方药均能明显(1)稳定MMP,益气健脾方药更具优势;(2)抑制大脑皮质神经元密度的下降(对海马神经元密度的作用不显著)。益气健脾法方药还能明显降低神经元凋亡百分比(益气健脾祛痰化瘀法作用不显著)。结论益气健脾和益气健脾祛痰化瘀方药均有一定的神经元保护作用,单纯益气健脾方药作用更为显著。Objective: Comparative on Effect of Receipe for Replenishing spleen Qi and Replenishing Spleen Qi added with Dispelling Phlegm and Stasis on Brain-aging Mouse neuron protecting.Methods: Duplicate subacute senescense and brain aging animal model was made with D-galactose (D-gal); Mitochondrial membrane potential (MMP) of cerebral neuron and percentage of cerebral neuron apoptosis were assayed by flow cytometry (FCM); Change of neuron density of cerebral cortex and hippocampus was assayed by pathological image analysis system.Results: Replenishing Spleen Qi added with Dispelling Phlegm and Stasis all could obviously effect on: (1) Stablizing neuron MMP,the Receipe of Replenishing spleen Qi has a better function. (2) Increasing density of cerebral cortex neuron(the effect on hippocampus neuron is not obvious).The Receipe of replenishing spleen Qi could additionally and obviously inhibit apoptosis percentage (receipe of replenishing spleen Qi added with dispelling phlegm and stasis is not obvious).Conclusion: Replenishing spleen Qi and Replenishing Spleen Qi added with Dispelling Phlegm and Stasis both can protect neuron, The receipe to Replenishing spleen Qi is obviously more effective.

关 键 词：益气健脾 益气健脾祛痰化瘀 脑老化 线粒体膜电位 细胞凋亡 神经元密度 

分 类 号：R285.5[医药卫生—中药学]