高温胁迫对黄瓜花药中Ca^(2+)分布、ABA含量及蛋白质合成的影响  

Effect of Heat Stress on Calcium Distribution, ABA Contents and the Synthesis of Proteins in Cucumber Anthers

作　　者：张玉华[1] 朱月林[2] 缪旻珉[1] 

机构地区：[1]扬州大学农学院园艺系,扬州225009 [2]南京农业大学园艺学院,南京210095

出　　处：《园艺学报》2005年第2期314-317,共4页Acta Horticulturae Sinica

基　　金：国家自然科学基金资助项目(30100123);江苏省自然科学基金项目(创新人才DK2002403)

摘　　要：经45℃处理1h后黄瓜热敏品系新泰密刺单核花粉期花药胞内Ca2+水平上升较耐热品系JY4快,但在温度恢复28℃后其钙稳态恢复较慢;45℃处理1h后新泰密刺花药内源ABA含量上升幅度比JY4大,恢复28℃后下降速度也较快;高温处理未能造成此期黄瓜花药新蛋白质的合成,但蛋白(MW70kD,pI5.7和MW45.5kD,pI5.6)的表达热激后大幅度上调,且在JY4中的表达水平显著高于新泰密刺。上述结果表明,Ca2+和ABA均参与了黄瓜花药对高温胁迫的反应调节;黄瓜单核花粉期花药中热激蛋白的合成至少已被部分抑制。Under 45℃ treatment, Ca 2+ precipitates in pollen and anther cells increase more quickly in cucumber(Cucumis sativus L.)heat-sensitive line Xintaimici than in heat-tolerant line JY-4. While the temperature recover to 28℃, the Ca 2+ precipitates quantity drop to the normal level more tardily in Xitaimici anther cells. Endogenous ABA contents in two line anthers also ascend significantly after 1 h 45℃ treatment, especially in Xintaimici. When the temperature return to 28℃, ABA in Xitaimici anthers step down to the control level, while JY-4 anthers can maintain a higher ABA content within 2 h. No any additional protein points can be seen in the IEF-SDS-PAGE maps of cucumber anthers under 45℃ stress comparing with 28℃, although the expression of some proteins, such as (MW 70 kD,PI 5.7) and (MW 45.5 kD,PI 5.6) were upregulated remarkably after heat treatment(even higher in JY-4 than in Xintaimici). The results indicated both Ca 2+ and ABA signals were involved in the response of cucumber anthers to heat-stress. The synthesis of HSPs in cucumber uninucleate-binucleate-pollen-phase anthers were at least in part inhibited under 45℃.

关 键 词：黄瓜 高温 花药 钙 ABA 蛋白质双向电泳 

分 类 号：S642.2[农业科学—蔬菜学]