人芽囊原虫感染者外周血T细胞亚群及膜白介素-2受体的检测  

Detection of mIL--2R and T lymphocyte subsets in PBMC of the patiend infected by Blastocystis hominis

作　　者：崔玉宝[1] 陈琳[1] 李朝品[1] 

机构地区：[1]安徽理工大学医学院,淮南市232001

出　　处：《热带病与寄生虫学》2003年第2期85-87,共3页Journal of Tropical Diseases and Parasitology

摘　　要：目的探讨人芽囊原虫感染者外周血T细胞亚群及膜白介素-2受体(mIL-2R)的变化。方法采用生物素一链霉亲和素(BSA)法对卵囊阳性患者进行外周血T细胞亚群、mIL—2R检测。结果人芽囊原虫卵囊阳性者CD_3^+、CD_4^+、CD_8^+和CD_4^+/CD_8^+阳性百分率分别为(65.83±6.55)％、(43.55±6.10)％、(28.43±4.32)％和 1.58±0.32,静息期与诱导期mIL-2R表达水平分别为(2.92±1.06)％和(33.45±2.31)％;人芽囊原虫卵囊阴性者CD_3^+、CD_4^+、CD_8^+和CD_4^+/CD_8^+阳性百分率分别为(55.87±7.23)％、(39.26±6.43)％、(30.04±5.67)％和1.36±0.41,静息期与诱导期mIL—2R表达水平分别为(4.31±1.47)％和(35.41±3.12)％,两者相比,差异均有显著性(P<0.05～0.01)。结论人芽囊原虫感染与细胞免疫功能密切相关,T细胞亚群、mIL-2R在抗人芽囊原虫感染中起重要作用。Objective To probe into possible changes of cellular immune function in the patients in- fected by Blastocystis hominis. Methods Biotin-Streptavidin (BSA) was used to detect T lymphocyte subsets and membrane interleukin-2 receptor (mIL-2R) in peripheral blood mononuclear cell (PBMC) of the patients infected by Blastocystis hominis respectively. Results In patients whose stools were found Blastocystis hominis oocyst in, the positive rates of CD_3^+,CD_4^+ and CD_8^+ were (65.83±6.55)%, (43.55±6.10)%, (28.43±4.32)%, the ratio of CD_4/CD_8^+ was 1.58±0.32, and the level of mIL-2R in silence and induction phage were (2.92± 1.06)% and (33.45±2.31)% respectively. On the other hand, in patients whose stools were not found Blasto- cystis hominis oocyst in, the positive rates of CD_3^+、CD_4^+ and CD_8^+ were (55.87±7.23)%, (39.26±6.43)%, (30.04±5.67)% ,the ratio of CD_4^+/CD_8^+ was 1.36±0.41, and the level of mIL-2R in silence and induction were (4.31±1.47)% , (35.41±3.12)% respectively. Statistics found significant difference between the two groups (P<0.05～0.01). Conclusions In the patients infected by blastocystis hominis, cell mediated immune took part in resisting the parasite, and T lymphocyte subsets and mIL-2 played an important role.

关 键 词：人芽囊原虫 原虫感染 外周血 T细胞亚群 膜白介素-2受体 细胞免疫 

分 类 号：R531[医药卫生—内科学]