高效毛细管电泳测定脑脊液中的胱氨酸  

Determination of Cystine in Cerebral Spinal Fluid by High Performance Capillary Electrophoresis

作　　者：王荣[1] 贾正平[1] 朱公建 谢华[1] 张强[1] 马骏[1] 费改顺[1] 熬燕[1] 

机构地区：[1]兰州军区兰州总医院全军临床药理基地,兰州730050 [2]甘肃省医学科学院实验分析中心,兰州730050

出　　处：《药物分析杂志》2005年第5期544-546,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：甘肃省自然科学基金(ZS031-A25-70-E)

摘　　要：目的:建立了高效毛细管电泳快速测定脑脊液中胱氨酸的方法,以满足临床诊断和治疗。方法:在碱性条件下,2,4-二硝基氟苯(DNFB)与胱氨酸反应生成 DNFB 氨基酸衍生物,在四硼酸钠缓冲液(pH 9.8)中电泳分离,电压28kV,检测波长340nm。结果:胱氨酸 DNFB 衍生物可在4min 内分离测定,在0.208-166.4μmol·L^(-1)范围内呈线性,r=0.9984,最低检测浓度0.1μmol·L^(-1),平均回收率98.25%,日内、日间精密度小于3.0%(n=5)。结论:该方法快速、简便、灵敏,适用于临床药物浓度测定及相关研究。Objective:A method for the determination of cystine by capillary electrophoresis was established. Methods:The assay was based on precolumn derivatization of cystine(Cys)with 2,4-dinitrofluorobenzen(DNFB) in the phosphate buffer system(pH9.5),the quantitative analysis was performed with external standard under the optimal conditions.Results:After simple treatment of the samples,separation and determination of Cys were ob- tained within 4 minutes.The calibration curve of cystine showed good linearity in the range of 0.208-166.4μmol ·L^(-1) with r=0.9984,and the detection limit was 0.1μmol·L^(-1).Conclusion:The method can be used for the routine cerebral spinal fluid for the clinical and scientific objective.

关 键 词：高效毛细管电泳 定脑脊液 胱氨酸 氨基酸衍生物 药物浓度测定 诊断和治疗 二硝基氟苯 平均回收率 碱性条件 电泳分离 检测波长 分离测定 检测浓度 相关研究 缓冲液 精密度 临床 

分 类 号：R284.1[医药卫生—中药学] R446.14[医药卫生—中医学]