GC-MS联用技术测定广藿香油中广藿香酮的含量  

GC-MS Assay for the Determination of Pogostone in Essential Oil of Herba Pogostemis

作　　者：汪小根 邹玉繁[2] 王玉生[2] 

机构地区：[1]广东省中药研究所,广州510520 [2]广东化工制药职业技术学院,广州510520

出　　处：《药物分析杂志》2005年第5期582-584,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：广东省中医药局2004年度滚动资助项目

摘　　要：目的:建立1种测定广藿香油中广藿香酮含量的方法。方法:以十八烷为内标,GC-MS 联用仪作为检测器,采用程序升温,初始温度120℃,维持3min 后以10℃·min^(-1)升温至220℃,维持5min,分流比为1∶50。结果:广藿香酮在0.5-2.5μg范围内呈良好的线性关系(r=0.9992),平均回收率为99.3%。结论:本法准确,特异性强,重复性好,可用于广藿香油的质量监控。Objective:To develop a new method for the determination of pogostone in essential oil of Herba pogoste- mis.Method:The essential oil was analyzed quantitatively on GC-MS instrument using n-octadecane as the in- ternal standard and high purity helium as the carries gas;The temperature was advanced by degrees,initial tempera- ture was 120℃ remaining 3 minutes,then heightened 10℃ per minute,the final temperature was 220℃ remai- ning 3 minutes.Results:There was a good linear relationship in the range of 0.5-2.5μg pogostone(r=0.9992). The average recovery was 99.3%,RSD=1.22%.Conclusion:The method is accurate,of good specificity and re- producibility,It can control the quality of essential oil of Herba pogostemis.

关 键 词：GC-MS联用技术 广藿香酮 广藿香油 含量 测定 平均回收率 程序升温 线性关系 质量监控 十八烷 检测器 5μg 特异性 重复性 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]