RP-HPLC法测定维胺酯乳膏中维胺酯的含量  

RP-HPLC Determination of Viaminate in Viaminate Cream

作　　者：曾令高 龚士学 曾晓莉 袁佩[2] 

机构地区：[1]重庆市药品检验所,重庆400015 [2]重庆医科大学,重庆400016

出　　处：《药物分析杂志》2005年第5期585-586,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:测定维胺酯乳膏中维胺酯的含量。方法:采用 RP-HPLC,Kromasil C_(18)(4.6mm×250mm,5μm)色谱柱,甲醇-水(93∶7)为流动相,检测波长371nm。结果:维胺酯在2.068-20.68μg·mL^(-1)范围内线性关系良好(r=0.9999),平均回收率98.72%(n=5),方法精密度 RSD=0.9%。结论:方法简便、快速,准确。Objective:To determine the effective contents of viaminate in viaminate cream.Methods:RP-HPLC with Kromasil C_(18)(4.6mm×250mm,5μm)column was used for the determination,the mobile phase was metha- nol-water(93:7)detection was with absorption at 371 nm.Results:The calibration curve was linear between 2.068-20.68μg·mL^(-1)(r=0.9999).The average recovery was 98.72%(n=5)and RSD=0.9%.Conclu- sion:This method is simple,rapid and accurate.

关 键 词：RP-HPLC法测定 维胺酯乳膏 含量 平均回收率 检测波长 线性关系 色谱柱 流动相 精密度 

分 类 号：R284.1[医药卫生—中药学] R758.733[医药卫生—中医学]