塞来昔布抑制荷人肺癌裸鼠肿瘤的实验研究  

An experimental study on the inhibitive effect of celecoxib on tumor in nude mice bearing lung cancer xenografts

作　　者：郑建[1] 周丹[2] 陈鸿义[2] 刘桐林[2] 李简[2] 李维青[2] 

机构地区：[1]武警江苏总队医院外科,江苏扬州225003 [2]北京大学第一医院胸外科,北京100034

出　　处：《武警医学院学报》2005年第4期271-273,F004,共4页Acta Academiae Medicinae CPAPF

摘　　要：【目的】研究塞来昔布对荷人肺癌裸鼠肿瘤的抑制作用。【方法】采用人肺癌A549细胞系体外培养后进行裸鼠移植瘤实验。通过移植瘤重、微血管密度和癌细胞凋亡指数比较,观察塞来昔布干预对荷人肺癌裸鼠肿瘤的抑制作用。【结果】对照组瘤重平均(2.1567±0.9750)g,实验组平均(0.9783±0.5423)g,两组有显著性差异(P<0.05),抑瘤率为54.64%;移植瘤微血管密度对照组为(42.82±10.25)个/HP,实验组为(22.24±5.62)个/HP(P<0.05),癌细胞凋亡指数对照组为1.58±0.61,实验组9.50±2.51(P<0.05)。【结论】塞来昔布对荷人肺癌裸鼠肿瘤有抑制作用,其作用与抑制肿瘤血管生成和促进肿瘤细胞凋亡相关。ObjectiveTo observe the inhibitory effect of a nonsteoidal anti-inflammatory drugs,celecoxib,a selective inhibitor of cyclooxygenase-2(COX-2),on tumors in nude mice bearing lung cancer xenografts.MethodsNude mice bearing lung cancer xenografts were administered with celecoxib,and then the weight of implanted tumors was measured.MVD of implanted tumors was monitored with immno-histochemistry.The apoptotic cells in implanted tumors were stained by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling(TUNEL) technique.ResultsThe average weight of tumor nodules in control group was (2.15670.9750) g,while in the experimental group it was (0.97830.5423) g,with the inhibitive rate being 54.64%.There is statistical difference(P<0.05).The MVD of transplanted tumor in the experimental group manifested a significant decrease compared with control(P<0.05).Apoptosis of transplanted tumor cells showed that apoptotic index(AI) of the control group was 1.580.61,while in the experimental group it was 9.502.51,showing significant difference (P<0.05).ConclusionCelecoxib showed suppressive effect on tumors in nude mice bearing lung cancer xenografts.Induction of angiogenesis and apoptosis might be its important mechanisms.

关 键 词：肺癌 塞米昔布 微血管密度 凋亡指数 

分 类 号：R979.1[医药卫生—药品]