大豆花叶病毒症状反应的遗传研究  

Studies on Inheritance of Symptom Reaction to Soybean Mosaic Virus in Soybean

作　　者：智海剑[1] 盖钧镒[1] 

机构地区：[1]南京农业大学大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室,南京210095

出　　处：《中国农业科学》2005年第5期944-949,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目(30170607);江苏省自然科学基金资助项目(BK2004100)

摘　　要：利用对SMV不同株系分别表现抗病(免疫或无症状)、坏死以及花叶的品种,配置花叶×抗病,花叶×花叶、花叶×坏死,坏死×坏死、坏死×抗病5类杂交组合。各组合的F1、F2和部分B1、F3世代在接种大豆花叶病毒Sa,SC8和N3株系的条件下,研究了大豆花叶病毒症状反应的遗传。结果表明,花叶×抗病组合接种SC8株系后,F1表现抗病,F2和B1群体分别发生3抗∶1感以及1抗∶1感的表型和基因型分离,说明一对等位基因控制大豆对SC8的抗病和花叶症状,其中抗病表现显性,花叶表现隐性。花叶×坏死组合接种SC8和Sa后,F1表现坏死,F2群体发生3坏死∶1花叶分离,说明控制坏死和花叶症状的基因是等位的,坏死基因对花叶基因表现为显性。坏死×抗病组合的F1表现抗病,F2出现3抗∶1坏死分离,说明抗病对坏死为显性。坏死×坏死,花叶×花叶两类组合后代接种不同株系后均没有发生症状分离,说明不同品种间控制花叶的基因是等位的;控制坏死的基因也是等位的。根据以上遗传试验推断,大豆对SMV的抗病(无症状)、坏死以及花叶3类症状由1组复等位基因控制,对应的等位基因可分别表示为SR、sN和sm,其中SR对sN和sm均表现显性,sN对sm表现显性。Five kinds of crosses (M×R, M×N, N×R, N×N and M×M,R, N and M representing resistant, necrotic and mosaic symptom, respectively) and their parents were used to study the inheritance of symptom reaction to soybean mosaic virus (SMV) in soybean. The results were as follows: (1) All plants of F1 from M×R crosses were symptomless. F2 populations segregated in a 3R: 1S ratio. The results showed that a single dominant gene controls resistance to SC8. (2) All F1 plants from M×N produced necrotic symptom, F2 population segregated in a 3N:1S ratio. It indicated that genes conditioning necrotic and mosaic symptom were at a same locus with necrosis being dominant to mosaic. (3) All F1 plants from N×R performed symptomless, F2 population segregated in a 3R : 1N ratio. It indicated that genes conditioning resistant and necrotic reaction are at a same locus with resistance being dominant to necrosis.(4)Non-segregation in N×N and M×M crosses showed that the genes for necrosis in different cultivars were at a same locus and the genes for mosaic symptom in different cultivars were also at a same locus. It was inferred that three types of symptoms of SMV in soybean were conditioned by a set of multiple alleles and the gene symbols SR, sN and sm were assigned for resistance, necrosis and mosaic symptom, respectively, with SR dominant to sN and sm and sN dominant to sm.

关 键 词：大豆 花叶病毒病 症状反应 抗性遗传 复等位基因 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治] S565.1[农业科学—植物保护]