Triton损伤成年大鼠嗅上皮对嗅球钙结合蛋白D和小白蛋白表达的影响  

Effect of olfactory epithelium lesion by Triton on expression of calbindin and parvalbumin in adult rat olfactory bulb

作　　者：秦照萍[1] 叶树明[1] 杜继曾[1] 沈公羽[1] 

机构地区：[1]浙江大学生命科学院玉泉校区生物系,浙江杭州310027

出　　处：《中国药理学与毒理学杂志》2005年第3期226-228,共3页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金资助项目 (30 0 70 190 )~~

摘　　要：目的　探讨嗅觉障碍的可能机制。方法　成年SD大鼠,TritonX 10 0灌流单侧鼻孔,30 ,4 5和6 0d后用免疫组化方法检测嗅球的钙结合蛋白 D(CB)和小白蛋白(PV)表达。结果　损伤后30d ,与对照侧相比,损伤侧嗅球的CB和PV阳性细胞密度减少6 8.9%和6 6 .7% ,4 5d后减少4 6 .4 %和5 0 .0 % ,4 5d的CB和PV阳性细胞密度比30d时增加,6 0d时接近对照侧。结论　大鼠嗅球中CB和PV的表达受Triton诱导的传入神经阻滞的可逆调控。AIM To discuss the possible mechanism of olfactory dysfunction. METHODS Adult Sprague Dawley rats were given a single intranasal irrigation with 100 μL 0.7% Triton X-100. At specific timepoint after treatment (30, 45 and 60 d, respectively), expression of calbindin(CB) and parvalbumin(PV) of olfactory bulb(OB) was exmined by immunohistochemistry. RESULTS As compared with control, densities of CB- and PV-immunoreactive cells in OB ipsilateral to lesioned naris on 30 d decreased by 68.9% and 66.7%, respectively, and by 46.4% and 50.0% on 45 d . Densities of CB- and PV-immunoreactive cells on 45 d were higher than that of 30 d, densities on 60 d were similar to that of the control. CONCLUSION Expression of CB and PV in adult rat OB was regulated reversibly by deafferentation induced by Triton.

关 键 词：嗅球 TRITON X-100 钙结合蛋白 小白蛋白 

分 类 号：R765[医药卫生—耳鼻咽喉科]