两种纯化苏云金杆菌HD-73毒素蛋白方法的比较研究  

Comparative study of two purification methods of Bacillus thuringiensis HD-73 toxin protein

作　　者：宋萍[1] 潘映红[2] 陆秀君[1] 王勤英[1] 张凤巧[3] 

机构地区：[1]河北农业大学植物保护学院,河北保定071001 [2]中国农科院植物保护研究所,北京100094 [3]河北农业大学图书馆,河北保定071001

出　　处：《河北农业大学学报》2005年第3期63-65,共3页Journal of Hebei Agricultural University

基　　金：国家自然科学基金重点项目(30130130);河北农业大学青年基金项目

摘　　要：以苏云金杆菌HD 73伴胞晶体为活性蛋白,经胰蛋白酶酶解后分别用阴离子交换柱分离纯化法和等电点纯化法进行纯化,纯化后得到的样品进行SDS-PAGE分析,结果表明,两种分离纯化都获得67kDa的毒素蛋白,但是离子交换柱纯化法样品消耗量大,得率低;而等电点沉淀法样品消耗少,得率高。Studies were carried out about purification of HD-73 toxin protein by two methods, toxin was converted by trypsin-treated from HD-73 pro-toxin, then purified by an anion-exchange column and pH dependent precipitation method. Samples of purified toxin were analyzed with SDS-PAGE. The results are as follows: 67 kDa toxin protein was obtained by two methods, but anion-exchange column has high wastage and low productivity, on the contray to other method.

关 键 词：BT CRY1AC 离子交换柱 等电点沉淀 SDS—PAGE 

分 类 号：S435[农业科学—农业昆虫与害虫防治]