人精浆蛋白双向谱型特征及其器官特异性的研究  

THE PRELIMINARY STUDY OF THE TWO-DIMENSIONAL ELECTROPHORETIC PATTERN OF HUMAN SEMINAL PROTEIN

作　　者：李剑平[1] 贾静涛[1] 刘春燕[1] 铁坚[1] 

机构地区：[1]中国医科大学法医血清学教研室,沈阳110001

出　　处：《中国法医学杂志》1994年第2期75-77,共3页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金

摘　　要：本文应用双向聚丙烯酰胺凝胶电泳（2D—PAGE）技术分析了60例正常人精浆中的蛋白成分。阐明了人精浆蛋白双向电泳谱型的特征，并将人精浆蛋白双向电泳谱型与其他体液蛋白双向谱型进行了比较，证明人精浆蛋白双向电泳谱型具有器官特异性。本文还用2D－PAGE对不同条件下保存的精斑进行了认定。并将其应用于强奸案的鉴定。Proteins of human seminal plasma obtained from 60 normal males were analyzed by two-dimen-sional polyacrylamide gel electrophoresis (2D-DAGE) in this study. The characteristics of two dimen-sional electrophoretic pattern of human seminal plasma was demonstrated by comparison of its electrophoretic pattern with other body fluids. Semen stains stored under different conditions were also identified by 2D-PAGE. This method was applied in a case.

关 键 词：人精浆 精斑 双向电泳 器官特异性 

分 类 号：R89[医药卫生—法医学]