趋化性细胞因子MIP-1α促小鼠外周血DC前体细胞动员的实验研究  

Mobilization of the dendritic cell precursors to circulation after administration of chemokine MIP-1α in mice

作　　者：吴雨岗[1] 汪良[2] 丘凌[1] 何宋兵[1] 杨桂林[1] 万兵[1] 张雁云[1] 

机构地区：[1]上海第二医科大学上海市免疫学研究所,上海200025 [2]苏州大学附属第一医院普外科,苏州215006

出　　处：《现代免疫学》2005年第3期191-195,共5页Current Immunology

基　　金：上海第二医科大学211工程资助项目

摘　　要：抗MIP-1α抗体可以阻断P.acnes对DC前体细胞的动员作用。对C57BL/6J(B6)小鼠静脉直接注射MIP-1α,24h后在B6小鼠外周血中F4/80-B220-CD11c+细胞明显增多,占外周血单个核细胞(PBMC)13.47%±1.4%。新鲜分离的F4/80-B220-CD11c+细胞不具有成熟DC的特征,经过细胞因子GM-CSF、IL-4和mTNF-α体外培养7d后的F4/80-B220-CD11c+细胞呈现树突状突起并形成细胞团簇,高度表达Ia、CD11c、DEC205、CD80、CD86,中度表达CD40,不表达CD8α、F4/80表面标志,并具有极强的刺激异源性T细胞增殖的能力。总之,研究表明趋化性细胞因子MIP-1α参与调节DC前体细胞的动员,并且注射MIP-1α可以直接迅速动员F4/80-B220-CD11c+DC前体细胞进入小鼠外周血。实验首次提出了用趋化性细胞因子MIP-1α动员DC前体细胞进入外周血的思路和实践,为体外获取大量功能正常的DC开辟了新的便捷途径。In the present study, the effect of the anti-MIP-1α antibody to block the recruitment of dendritic cell precursors induced by Propionibacterium acnes was found. The F4/80-B220-CD11c+ cells in the peripheral blood of C57BL/6(B6) mice were found to be obviously increased, accounting to 13.47%±1.4% of peripheral blood mononucleated cells(PBMC), when MIP-1α was directly introduced intravenously into B6 mice. The freshly isolated F4/80-B220-CD11c+ cells did not show any characters of mature dendritic cells (DC). However, when this type of cells was cultivated in the presence of GM-CSF, IL-4 and mIFN-γ for 7 days, they could differentiate into mature DC that possessed several characteristics of mature DC, such as dendrites and aggregate formation in morphology, high level expressions of Ia, CD11c, DEC205, CD80 and CD86 as well as moderate level expressions of CD40 without any expression of CD8α or F4/80 in phenotype, gain of the capacity to stimulate allogeneic T cells. It is concluded that MIP-1α participates in regulating the recruitment of the DC precursors, in which a single administration of it can rapidly recruit DC precursor into circulation. This study open a new approach to obtain large amount of DC with normal functions within a short time.

关 键 词：树突状细胞 MIP-1Α 动员 

分 类 号：R392.11[医药卫生—免疫学]