不同制剂诱导单个核细胞IL-23、IL-12亚基表达的对比分析  

A comparative analysis on the expressions of IL-23 and IL-12 subunits on peripheral blood mononuclear cells induced by different inducers

作　　者：马小彤[1] 葛薇[1] 张秀军[1] 徐玢[1] 林永敏[1] 李戈[1] 宋玉华[1] 吴克复[1] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所血液病医院实验血液学国家重点实验室,天津300020

出　　处：《现代免疫学》2005年第3期213-216,共4页Current Immunology

基　　金：国家自然科学基金资助项目(30471964)

摘　　要：为探索并比较人类外周血单个核细胞(PBMC)IL-23、IL-12亚基表达的诱导剂和调控模式,分离正常人PBMC、单核细胞、CD4+、CD8+T淋巴细胞亚群,检测各种细菌、细菌产物、细胞因子、丝裂原对上述细胞IL-23和IL-12亚基表达的诱导作用,半定量RT-PCR方法测定各亚基的mRNA表达水平。结果显示,未经刺激的PBMC可表达IL-23p19和IL-12p35mRNA;脂多糖(LPS)和金黄色葡萄球菌(SAC)可上调PBMCIL-23和IL-12各亚基的表达;γ干扰素(IFN-γ)预处理后予LPS刺激可使单核细胞IL-23p19、IL-12p35和IL-12p40表达高于LPS直接作用的表达水平。植物血凝素(PHA)可促进CD4+、CD8+T细胞IL-23p19表达,而对IL-12p35、p40表达无调节作用。本文结果提示,IL-23的两个亚基的表达都处于紧密调控之下,易受各类诱导剂调节;人类外周血细胞IL-23p19的表达和调控模式与IL-12p35既有相似之处又存在不同点。To investigate the expressions of IL-23 and IL-12 subunits on peripheral blood mononuclear cells (PBMC) induced by different inducers and their regulation patterns, human PBMC, monocytes and the CD4+ and CD8+ T lymphocytes were isolated, and the effects of various bacteria, bacterial products cytokines and mitogens on the expressions of IL-23 and IL-12 subunits on these cells were evaluated by semi-quantitative RT-PCR. The results showed that IL-23 p19 and IL-12 p35 mRNA were expressed in fresh preparations of PBMC, and the expression of all the IL-23 and IL-12 subunits were up-regulated by lipopolysaccharide (LBS) and Staphylococcus aureus Cowan strain (SAC). The expression levels of IL-23 p19, IL-123 p35 and IL-12 p40 on the LPS-stimulated monocytes pretreated with IFN-γ were higher than those stimulated with LPS alone. PHA promoted the expressions of IL-23 p19 on CD4+ and CD8+ T cells, whereas the expressions of IL-12 p35 and p40 were minimally regulated by PHA. Our results suggest that both subunits of IL-23 are under tight regulation, and easily regulated by various regulators,the expression pattern and regulation mode of IL-23 p19 is similar to as well as distinct from that of IL-12 p35.

关 键 词：白介素23 白介素12 γ干扰素 植物血凝素 

分 类 号：Q256[生物学—细胞生物学]