用地高辛配基标记的pAW101探针杂交分析DNA多态性及其应用  

RFLPs ANALYSIS OF DIGOXIGENIN-LABELLED pAW101 PROBE AND ITS PRACTICAL USES

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作  者:罗超权[1] 杨英浩[1] 伍新尧[1] 罗健扬 刘超[2] 

机构地区:[1]广州中山医科大学,广州510089 [2]广州市刑事科学技术研究所,5130300

出  处:《中国法医学杂志》1994年第3期134-137,共4页Chinese Journal of Forensic Medicine

摘  要:将NaCl盐析法抽提人基因组DNA与地高辛配基标记DNA探针的方法相结合,检测了15个家系子代与亲代DNApAW101-EcoRI限制性片段的遗传关系。结合所测同工酶(EsD.GLOI.pGM;.Acp)的基因频率、按Essen-moller氏公式计算,父权概率均达到99.73%以上。本法经济、有效、实用、易于在国内普遍实验室开展。A new method to revel RFLPs is presented. The human genomic DNAwas purified by saturatedNaCl solution and the pAW101 probe labelled with digoxigenin-dUTP. The relationships of RFLPsand genetic patterns of PGM1 (phosphoglucomutase),EsD (esterase D),GLO1 (glyoxalase)and ACP(acid phosphatase ) between the fillal generation and parental generation were detected in 15 families(among them 11 cases were aborted fetuses). The probability of paternity (w)was caculated accor-ding to Essen - Moller's formula, each w vlua was over 99. 73 %, reached the standard of incladingpaternity. An effective,rapid, and non-toxic RFLPs technique was established, which is easy to man-age in common lab oratories.

关 键 词:限制性 片段长度多态性 地高辛配基 亲权鉴定 

分 类 号:R89[医药卫生—法医学]

 

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