蝴蝶兰叶片诱导植株再生的研究  

Plantlets Regeneration from Leaf Explants of Phalanopsis

作　　者：李杰[1] 黄敏仁[1] 王明庥[1] 蔡汝 

机构地区：[1]南京林业大学林木遗传和基因工程国家林业局重点实验室,江苏南京210037 [2]江苏省兰花工程研究中心,江苏泰州225321

出　　处：《南京林业大学学报（自然科学版）》2005年第3期28-32,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江苏省科技攻关计划项目(BE2003384);江苏省"六大人才高峰"项目(苏人发[2004]28号)

摘　　要：以蝴蝶兰叶片为外植体,建立了蝴蝶兰植株再生系统。结果表明:(1)可通过花梗节芽建立丛生芽诱导体系,获得大量无菌材料,丛生芽诱导增殖的最佳激素组合为7.5 mg/L 6 BA+1.0 mg/L NAA;(2)植物激素显著影响叶片类原球体的诱导,在10.0 mg/L 6 BA+1.0 mg/L NAA的激素组合处理下,各品种的诱导效果最好;(3)4种基本培养基中,1/2MS和Hyponex对类原球体的增殖和分化效果较好;(4)蔗糖浓度对类原球茎分化成苗的影响可用y=-0.019 92x2+0.077 51 x+0.17 进行拟合,在蔗糖浓度为20 g/L时,蝴蝶兰类原球茎分化率达最高。Plantlets regeneration from leaf explants of Phalanopsis were studied.The results showed that:(1)Decontamiated-materials could be obtained by inducing from floral stalk,and proliferation of plantlet was achieved on Murashige and Skoog(MS) medium supplemented with 6-BA(7.5mg/L)+NAA(1.0mg/L);(2)6-BA and NAA can stimulate PLBs(protocorm-like bodies) formation and the effective ruslts could be obtained on MS 6-BA(10.0mg/L)+NAA(1.0mg/L);(3)The optimal mediums were 1/2MS and Hyponex mediums for proliferation and transformation of PLBs;(4)The relations between the concentration of sucrose and plantlets regenerations from PLBs could be formulated by y=-0.01992x^2+0.07751x+0.17000,and plantlets developed well on 1/2 MS containing 20g/L without any growth regulators.

关 键 词：蝴蝶兰 叶片 植株再生 

分 类 号：S682.31[农业科学—观赏园艺]