钩吻素子治疗银屑病样动物模型的疗效观察  

Therapeutic effects of koumine on psoriasis: an experimental study in mice

作　　者：张兰兰[1] 黄昌全[1] 张忠义[1] 王志睿[1] 林敬明[1] 

机构地区：[1]南方医科大学珠江医院药剂科,广东广州510282

出　　处：《第一军医大学学报》2005年第5期547-549,共3页Journal of First Military Medical University

基　　金：广东省科技攻关项目(C30801)~~

摘　　要：目的观察钩吻素子(Kou)对银屑病动物模型的疗效。方法采用鼠尾鳞片表皮和鼠阴道上皮实验模型,不同组别的小鼠给以甲氨蝶呤或不同浓度的Kou,分别观察其对上皮细胞分裂和表皮细胞分化的影响,以及对血清IL-2含量的影响。结果Kou中、高剂量组能显著抑制阴道上皮细胞有丝分裂、促进鼠尾鳞片表皮颗粒层的生成;Kou低、中、高剂量组均可明显降低小鼠血清IL-2的水平。结论提示Kou可能通过抑制小鼠表皮细胞增殖与促进其分化并减少炎性因子的生成而发挥作用,为其用于银屑病的治疗提供了实验依据。Objective To study the therapeutic effects of koumine on psoriasis in mouse models. Methods The effects of koumine on epithelial cell mitosis and epidermal cell differentiation was evaluated by collecting the samples of the vaginal mucous and squamous epidermis at the tail of mice treated with methotrexate or koumine at different doses. The levels of interleukin (IL)-2 were detected with enzyme-linked immunosorbent assay. Results High and intermediate doses of koumine showed remarkable inhibitory effect on mouse vaginal epithelial cell mitosis and promoted the formation of epidermal granular layer in the scales at the mouse tail. Three concentrations at 6, 30, 150 mg/kg of koumine decreased serum IL-2 level in the mice. Conclusion The therapeutic effect of koumine against psoriasis is related to the inhibition of epidermal cell proliferation, promoting the formation of granular cells and decreasing the serum level of IL-2.

关 键 词：钩吻素子 银屑病 药效学 

分 类 号：R285.5[医药卫生—中药学]