碱性淀粉酶菌株的紫外线和He-Ne激光复合诱变及其产酶条件的研究  

Study on the breeding of alkaline amylase producing strain by UV and He-Ne laser compound mutation and the conditions for enzyme production

作　　者：刘建龙[1] 刘建军[2] 杨连生[1] 

机构地区：[1]华南理工大学食品与生物学院,广州510641 [2]山东省食品发酵工程重点实验室,济南250014

出　　处：《激光杂志》2005年第3期83-84,共2页Laser Journal

基　　金：山东省科技攻关计划课题 (95 0 7)

摘　　要：以嗜碱芽胞杆菌BC -A3 6为出发菌株,通过紫外线(UV)、亚硝基胍(NTG)及激光复合诱变处理,选育出一株目前高产碱性淀粉酶的突变株JG -5 7,在摇瓶发酵培养基上3 5℃培养45h ,测得酶活力稳定在867u/ml左右,为出发菌株酶活力的2 .1倍。该菌株产酶适宜的pH值为9-10。By compound treatment with UV,TNG and laser to the initial strain BC-A36,the mutant JG-57 with high alkaline amylase activity was obtained.In the flask culture with fermentation medium at 35℃ for 45 hours its enzyme activity can reach 867u/m l ,which was 2.1 times greater than the initial strain.The alkaline amylase produced by the mutant JG-57 has strong adaptability to high pH of 9-10.

关 键 词：嗜碱芽胞杆菌 激光复合诱变 碱性淀粉酶 产酶条件 

分 类 号：TN256[电子电信—物理电子学]