MPPa光动力作用诱导人鼻咽癌细胞凋亡的实验研究  

Study of MPPa-mediated photodynamic therapy lnducing human nasopharyngeal carcinoma cells into apoptosis

作　　者：许川山[1] Albert Wing Nang Leung 

机构地区：[1]重庆医科大学超声影像学研究所 [2]Department of Chinese Medicine Education Division,School of Chinese Medicine,Hong Kong Baptist University,Hong Kong.,China

出　　处：《激光杂志》2005年第3期89-90,共2页Laser Journal

基　　金：国家自然科学基金资助 (编号 :3 0 3 0 0 468);香港浸会大学FRG课题资助

摘　　要：为观察MPPa光动力作用对鼻咽癌细胞凋亡的影响,应用AnnexinV—PI双染结合流式细胞仪分析MPPa光动力作用后人鼻咽癌细胞株CNE2细胞发生凋亡和继发性坏死的比率。结果显示MPPa光动力作用实验组人鼻咽癌细胞株CNE2细胞发生凋亡和继发性坏死的比率分别增加到16.43 %和4.64 % ,且均显著高于单纯光照射组、单纯MMPa光敏剂处理组和假照射组(P <0 .0 1) ,而三对照组间无明显差异(P >0 .0 5 )。表明MPPa光动力作用能有效诱导低分化人鼻咽癌细胞株CNE2细胞凋亡的发生。这也可能是MPPa光动力作用杀伤鼻咽癌的重要机制之一。To observe the effect of MMPa-mediated photodynamic therapy on apoptosis of human nasopharyngeal carcinoma cells,the apoptotic and secondary necrotic rate of the NPC CNE2 cells induced by MMPa-mediated photodynamic therapy was determined by flow cytometry with Annexin V-PI staining.The results showed that the apoptotic and secondary necrotic rate of the NPC CNE2 cells after photodynamic therapy significantly increased to 16.43% and 4.64%,and they were higher than those of the controls respectively(P<0.01).but there is no significance of the apoptotic and secondary necrotic rate between the controls(P>0.05).Our data demonstrate that MMpa-mediated photodynamic therapy might obviously trigger the NPC CNE2 cells into apoptosis,which might be one of the mechanisms of MMPa-mediated photodynamic therapy on nasopharyngeal carcinoma.

关 键 词：激光 MMPa 光动力治疗 鼻咽癌细胞 凋亡 

分 类 号：TN248.1[电子电信—物理电子学]