苗龄与6-BA浓度对大豆子叶节丛生芽诱导的影响  

Effect of Aseptic Seeding Age and 6-BA Concentration on Overgrowing Shoots of Soybean Cotyledonary Node

作　　者：龚学臣[1] 季静[1] 王萍[2] 吴颖[3] 王罡[1] 

机构地区：[1]天津大学农业与生物工程学院 [2]淮海工学院海洋学院,连云港222005 [3]吉林大学农学部,长春130062

出　　处：《吉林农业大学学报》2005年第2期128-130,共3页Journal of Jilin Agricultural University

基　　金："国家植物转基因技术研究开发与中试基地建设"专项课题资助项目(J99-B-001);农业部作物遗传育种重点实验室资助项目

摘　　要：以4个栽培大豆品种的子叶节为外植体,进行了苗龄与6 BA浓度对大豆子叶节丛生芽诱导影响的试验研究。结果表明:大豆子叶节丛生芽率在不同大豆品种、无菌苗苗龄和6 BA浓度间都存在着极显著的差异。诱导子叶节丛生芽产生的适宜苗龄吉林30和黑农35为8d,吉林35和合丰25为12～16d;6 BA的适宜质量浓度为1 5mg/L。<Abstrcat> Using cotyledonary nodes of four soybean varieties as explants, the factors affecting overgrowing shoots of soybean cotyledonary node were investigated in this research.The results showed that the inducing rates of overgrowing shoots were significantly different among different plant genotypes, ages of aseptic seedlings and concentrations of 6-BA. The appropriate ages of aseptic seedlings induced by overgrowing shoots of cotyledonary node of different genotypes were as follows: Jilin 30 and Heinong 35 are (8　d); Jilin 35 and Hefeng 25 are 12～16 d. The appropriate concentration of 6-BA is 1.5　mg/L.

关 键 词：大豆 子叶节 6-BA 无菌苗苗龄 丛生芽 

分 类 号：S565.1[农业科学—作物学]