冷冻保存同种异体骨-ACL-骨移植后止点转归研究  

Biomechanics and Histology of the Fate of Autograft Versus Allograft in Anterior Cruciate Ligament Reconstruction

作　　者：张长春[1] 周建生[1] 肖玉周[1] 刘振华[1] 官建中[1] 

机构地区：[1]蚌埠医学院附属医院骨科,安徽蚌埠233000

出　　处：《解剖与临床》2005年第2期104-107,共4页Anatomy and Clinics

摘　　要：目的:比较自体骨-前交叉韧带(ACL)-骨移植和冷冻保存同种异体骨-ACL-骨移植重建ACL后,韧带止点的组织学和生物力学转归。方法:将日本大耳兔和新斯兰兔各60只随机分成自体骨-ACL-骨移植组和二步冷冻保存同种异体骨-ACL-骨移植组。术中及术后均不使用免疫抑制剂。术后4、8、12周分别切取ACL作大体、组织学观察和生物力学测试。结果:自体骨-ACL-骨移植组和冷冻保存骨-ACL-骨移植组在4周时止点处组织学上已有纤维组织与骨相连,但未见“潮线”形成,而在12周时两组均有潮线形成;4周拉伸时,止点处断裂分别为60%、66.7%,而8周分别为13.3%、13.3%,12周时则分别为6.7%、6.7%。结论:两组在止点处有相似的生物力学性能和组织学愈合过程。Objective:To compare the histological and biomechanical results of the anterior cruciate ligament reconstruction with bone-ACL-bone autograft and cryopreserved bone-ACL-bone allograft.Methods:Sixty New Zealand rabbits and sixty Japanese rabbits were randomly divided into autograft group and two-step cryopreserved ACL allograft group.Immunosuppressants were not used after transplantation.The biomechanics. histology and morphology of the transplanted ACL were examined in the 4th,8th,and 12th week after transplantation.Results:In the 4th week postoperatively,the fibrous tissue was present in the interspace of the graft and the bone tunnel of both grafts and discontinuous tidemark was not formed;but in the 12th week,discontinuous tidemark was demonstrated in both grafts.The ligament-cartilage insertion of the autografted and allografted ACL was torn in 60% and 66.7% in the 4the week,13.3%,13.3% in the 8th week and 6.7%,6.7% in the 12th week,respectively.Conclusion:Two-step cryopreserved bone-ACL-bone allograft is similar to bone-ACL-bone autograft in the biomechanics and histology of ligament-cartilage insertion.

关 键 词：同种异体骨 冷冻保存 转归 移植后 生物力学测试 生物力学性能 骨移植 前交叉韧带 日本大耳兔 免疫抑制剂 自体骨 组织学 12周 韧带止点 纤维组织 愈合过程 ACL 术后 潮线 

分 类 号：R687.3[医药卫生—骨科学]