维生素A和锌联合在体外诱导人外周血单核细胞DNA损伤的研究  

Combination effects of vitamin A and zinc on human's PMBC DNA damage in vitro

作　　者：李婷欣[1] 李云[1] 鹿子龙[1] 

机构地区：[1]四川大学华西公共卫生学院,成都610041

出　　处：《卫生研究》2005年第3期319-322,共4页Journal of Hygiene Research

基　　金：国家自然科学青年基金资助 (No.3960 0 1 2 2 )

摘　　要：目的　通过碱性单细胞电泳凝胶技术和流式细胞术,研究维生素A和锌联合在体外诱导人的外周血单核细胞(PBMC)DNA的损伤。方法　分离健康人PBMC在体外培养,分别加入不同剂量的维生素A和锌改变培养基环境,通过单细胞电泳凝胶技术和流式细胞术,分别检测各个剂量组PBMC的细胞凋亡率与DNA断裂情况。结果　体外培养时补充适量的锌和维生素A尚未使PBMC凋亡率增加,没有加重DNA损伤,但同时补充过量的锌和维生素A就会引起PBMC的广泛凋亡和严重的DNA损伤(P <0 .0 5 ) ,且比由于维生素A或锌单独过量引起的损伤更严重。当维生素A过量时,补充适量锌对已损伤的细胞有一定的修复作用,但可能会扩大受损的细胞范围;当锌过量时,补充适量维生素A对正常细胞有一定的保护作用,但可能会加剧已受损细胞的损伤程度。结论　在体外试验中,过量的补充维生素A和锌可能会造成人的PBMC的DNA单链严重损伤,具体机理尚须进一步研究。Objective To study the combination effects of vitamin A (Vit.A) and zinc on human's PBMC DNA damage in vitro. Methods The PBMC from healthy volunteer was purification and cultured in the basic culture medium with Vit.A of 10 -4, 10 -5 and 10 -6mol/L, or/and with Zn of 10 -6, 10 -5, 10 -4 and 10 -3mol/L. The basic culture medium with K_2Cr_2O_7 of 1mmol/L was the male control. The damage of PBMC DNA was respectively examined by SCGE and the image analyzer measured the comet tail rate and length. The apoptosis of each group PBMC was detected by FCM. Results The overdose supplement of zinc and Vit.A can damage the PBMC DNA in vitro culture (P<0.01), while the suitable supplement doesn't find. When the Vit.A supplement has been excessive, giving suitable zinc could repair the damage of DNA, but would expand the injured cell. While when the zinc supplement has been excessive, giving suitable Vit.A could protect the healthy cells, but would aggravate the damage degree of injured cell. Conclusion In vitro culture, the overdose Vit.A and zinc supplement could damage the human's PBMC DNA significantly. This need more researches to provide.

关 键 词：维生素A 锌 单核细胞 DNA损伤 彗星试验 流式细胞术 细胞凋亡 

分 类 号：Q562[生物学—生物化学] Q581