慢性丙型肝炎患者外周血单个核细胞HCV RNA检测及其与机体免疫功能的相关性研究  

Hepatitis C viral RNA level in peripheral blood mononuclear cells and peripheral blood CD3^+ and CD4^+ lymphocyte subsets in patients with chronic hepatitis C.

作　　者：林雪松 罗锐 阎飞 

机构地区：[1]大连市第六人民医院,116001

出　　处：《实用肝脏病杂志》2005年第3期136-137,共2页Journal of Practical Hepatology

摘　　要：目的观察慢性丙型肝炎患者外周血单个核细胞(PBMC)HCVRNA含量及其对T淋巴细胞亚群的影响,以探讨HCV感染者PBMC中HCVRNA水平及其与机体免疫功能的关系。方法采用荧光定量PCR(FQPCR)技术对128例丙型肝炎患者血清、外周血单个核细胞的HCVRNA含量进行了检测,同时检测CD3+、CD4+、CD8+、CD4+/CD8+。结果PBMC内HCVRNA阳性组与HCVRNA阴性组比较,前者CD3+、CD4+水平降低、CD8+水平增高,CD4+/CD8+比值下降大于后者,差异有显著性(P<0.05)。结论丙型肝炎病毒侵染PBMC后可加重患者的细胞免疫功能紊乱。Objective To investigate hepatitis C viral RNA level in peripheral blood mononuclear cells (PBMC) and peripheral bolld CD3 + and CD4 + lymphocyte subsets in patients with chronic hepatitis C.Methods The hepatitis C viral RNA level in sera and in PBMCs were assayed by flow cytometrics.Results The serum HCV RNA was positive in 42.2%(54/128) of patients with chronic hepatitis C,while HCV RNA in PBMCs was positive in 41.4%(53/128).The peripheral blood CD3 + and CD4 + lymphocyte decrease and CD8 + cell increase in patients with PBMC positive HCV RNA were more obvious than in with negative HCV RNA ( P< 0.05).Conclusion The infection of HCV in PBMCs will lead to injury of cell immune function.

关 键 词：外周血单个核细胞 丙型肝炎患者 机体免疫功能 HCV 相关性研究 RNA检测 CD4^+/CD8^+比值 慢性 细胞免疫功能紊乱 T淋巴细胞亚群 RNA含量 CD3^+ PBMC 丙型肝炎病毒 荧光定量 同时检测 感染者 水平 显著性 

分 类 号：R512.63[医药卫生—内科学]