胎盘组织荧光PCR诊断早期先天梅毒的研究  

Study on detection of placental Treponema pallidum DNA for diagnosis of congenital syphilis by using FQ-PCR.

作　　者：彭石潜[1] 周华[2] 张荣[1] 温桂华[1] 

机构地区：[1]深圳市宝安区慢性病防治院,广东深圳518133 [2]深圳市慢性病防治院,广东深圳518020

出　　处：《中国热带医学》2005年第3期443-444,465,共3页China Tropical Medicine

摘　　要：目的评价胎盘组织荧光PCR(FQ-PCR)在先天梅毒早期诊断中的作用。方法对34例梅毒孕妇和30例非梅毒对照孕妇的胎盘和脐带组织进行梅毒螺旋体DNA的荧光定量PCR检测,并与常规临床及血清学综合诊断方法比较。结果34例梅毒孕妇的胎盘组织中,11例荧光PCR为阳性,阳性率为32.35%,30例非梅毒孕妇的胎盘和脐带样本荧光PCR均为阴性;以常规临床及血清学综合诊断方法为标准,则荧光PCR与常规方法结果的灵敏度为82%,特异性100%,约登指数0.82。显示在诊断先天梅毒新生儿时,FQ-PCR至少有不低于常规综合诊断方法的价值。结论与常规临床血清学综合诊断方法相比,荧光PCR均有明显的优势。Objective To evaluate the role of FQ-PCR in detection of placental Treponema pallidum DNA for diagnosis of congenital syphilis. Methods Thirty-four placental specimens from pregnant women with syphilis and thirty specimens from health pregnant women were blindly evaluated with: (1) FQ-PCR for placental Treponema pallidum DNA;(2) Traditional comprehensive approach for diagnosis of congenital syphilis. The results were compared with each other. Results Among the 34 placentas from syphilitic women who completed pregnancy, 11 had positive FQ-PCR. None of the 30 infants from health mother had positive result. FQ-PCR for placental Treponema pallidum DNA showed comparable sensitivity superior to the traditional comprehensive approach for diagnosis of congenital syphilis. Conclusion FQ-PCR for placental Treponema pallidum DNA could be an addition to conventional diagnostic tools.

关 键 词：先天梅毒 FQ-PCR 诊断方法 胎盘组织 荧光PCR 血清学 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]