去甲万古霉素载药涤纶血管材料的研制  

Preparation of norvancomycin-bonded dacron prostheses

作　　者：余维平[1] 景在平[1] 包俊敏[1] 赵志青[1] 赵珺[1] 梅志军[1] 

机构地区：[1]第二军医大学附属长海医院血管外科,上海200433

出　　处：《中华实验外科杂志》2005年第6期662-664,共3页Chinese Journal of Experimental Surgery

基　　金：军队杰出人才基金资助项目(98J005);上海市卫生系统"百人计划"基金资助项目(97BR047)

摘　　要：目的探讨去甲万古霉素载药涤纶血管材料(NV-DP)的制备,并对其形态特征、载药量、体外释药性能和有机溶剂残留量进行观察。方法以聚乳酸为载体采用乳剂溶剂挥发法制备去甲万古霉素聚乳酸微球;以乙烯醋酸乙烯共聚物为支撑剂将微球固载至涤纶材料制备NV-DP;采用高效液相色谱法(HPLC)测定去甲万古霉素(NV)含量和体外释药量;采用气相色谱法(GC)测定二氯甲烷残留量。结果微球平均粒径和载药量分别为30.7μm和(0.2533±0.0025)g/cm2;微球被成功地固载至涤纶材料,载药量为(0.24645±0.00061)g/cm2;NV-DP均匀,厚度为0.18mm;NV自NV-DP缓慢释放超过20d;二氯甲烷残留为0.0031%。结论NVDP壁薄,载药量大,在体外能够实现长时间释药,有机溶剂残留未超标。提示该载药血管材料适合用于制备抗感染血管移植物。Objective To explore the preparation of norvancomycin-bonded Dacron prosthesis (NV-DP) and evaluate its morphology,drug loading,in vitro release and organic solvent residue.Methods Norvancomycin-polylactic acid microspheres were prepared by emulsion and solvent evaporation method.Microspheres were bonded to Dacron prostheses using ethylene-vinyl acetate copolymer as support to make NV-DP.The drug loading and in vitro release of NV-DP were measured by HPLC.Dichloromethane in NV-DP was measured by GC.Results The mean diameter and drug loading of microspheres were 30.7 μm and (0.253?3± 0.002?5) g/cm^2,respectively.Microspheres were successfully bonded to Dacron prostheses with the drug loading of (0.246?45± 0.000?61) g/cm^2,and NV-DP was smooth with uniform thickness of 0.18 mm.Norvancomycin was released biphasically from NV-DP for over 20 days:in the initial phase (first 10 days),norvancomycin was rapidly released (80%) and,in the second phase,the rest was diffused gradually for more than 10 days.Dichloromethane residue was 0.0031%.Conclusion NV-DP had thin wall with high drug loading,sustained release effect,and limited solvent residue.The results suggested NV-DP is suitable for making infection-resistant vascular graft.

关 键 词：去甲万古霉素 载药涤纶血管材料 二氯甲烷 释药时间 血管移植物 

分 类 号：R318.08[医药卫生—生物医学工程]