辐射诱导犬胆管增殖平滑肌细胞凋亡中胱冬肽酶-3活性的影响  

Caspase-3 gene activity in radiation-induced dog biliary duct mucle cells apoptosis

作　　者：何贵金[1] 高沁怡[2] 许书河[3] 高红[4] 姜维国[5] 蒋涛[4] 戴显伟[1] 马凯[1] 

机构地区：[1]中国医科大学第二临床学院普外一科,沈阳110004 [2]中国医科大学第一临床学院同位素室 [3]中国原子能研究院同位素研究室 [4]中国医科大学第二临床学院小儿外科实验室,沈阳110004 [5]中国医科大学第二临床学院病理科,沈阳110004

出　　处：《中华实验外科杂志》2005年第6期686-688,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨γ射线对胱冬肽酶-3(Caspase-3)基因在犬胆管壁增殖平滑肌细胞凋亡中的活性影响。方法将103pd放射性支架和普通支架分别植入犬肝外胆管内,取出胆管标本,用免疫组化进行平滑肌细胞凋亡染色,并在JEM1200EX电镜下观察凋亡细胞超微结构变化;又用RTPCR检测γ射线诱导犬胆管壁凋亡的增殖平滑肌细胞中Caspase-3基因的mRNA。结果103pd支架组犬胆管组织中Caspase-3基因表达较普通支架组明显,且Caspase-3基因高表达组犬胆管出现明显增殖平滑肌细胞凋亡,而且犬肝外胆管无明显狭窄;而普通支架Caspase-3基因低表达,且犬胆管未出现增殖平滑肌细胞明显凋亡,而且犬肝外胆管有明显狭窄。结论辐射可以使Caspase3基因活性增高,切与细胞凋亡有关;103pd放射性支架通过激活Caspase-3基因,促进犬胆管增殖平滑肌细胞凋亡,可以抑制犬肝外胆管狭窄。Objective To research the Caspase-3 gene activity in γ-radiation-induced dog biliary duct mucle cell lines apoptosis.Methods Stenosis models of biliary duct after biliary duct intimal injury were established in dogs.The dogs were randomly divided into the ~ 103pd radioactive stent group and the control group.γ-radiation-induced apoptotic mucle cells was analysed by using immunohistochemical technique and their ultrastructural changes were observed under JEM-1200EX electron microscopy.The expression of Caspase-3 mRNA in the control andγ-radiation-induced apoptotic mucle cells was detected by RT-PCR.Resutls The expression of Caspase-3 mRNA was higher in ~ 103pd radioactive stent dog bile duct than in general stent dog bile duct,and apoptotic mucle cells were significantly increased in ~ 103pd radioactive stent dog bile duct than in general stent dog bile duct.Conclusion The high expression of Caspase-3 gene may aid the effects to understand the mucle cells sensitivity toγ-radiation apoptosis.~ 103pd radioactive stent may increase the expression of Caspase-3 gene in dog bile duct,and prevent the biliary narrow after dog bile duct was injured by balloon.

关 键 词：辐射诱导 犬 胆管增殖平滑肌细胞 细胞凋亡 胱冬肽酶-3 酶活性 Γ射线 

分 类 号：R815[医药卫生—放射医学]