骨形态发生蛋白-2对椎间盘细胞软骨特异性基因mRNA的调节作用  

Regulatory effect of bone morphogenetic protein-2 on expression of cartilage specificity gene mRNA in the human intervertebral discs

作　　者：王文波[1] 赵勇[1] 卢宇[1] 李雪松[1] 赵哲[1] 于长水[1] 

机构地区：[1]哈尔滨医科大学附属第一医院骨二科,150001

出　　处：《中华实验外科杂志》2005年第6期719-721,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目(30471741)

摘　　要：目的探讨骨形态发生蛋白(BMP)2对椎间盘细胞软骨特异性基因Sox9、Ⅱ型胶原和蛋白聚糖基因的调控作用。方法应用逆转录聚合酶链反应(RTPCR)技术检测BMP2对培养的人椎间盘细胞中Sox9、Ⅱ型胶原和蛋白聚糖基因mRNA表达的调控作用。结果在BMP2浓度为100μg/L(0.149±0.006,P<0.05)和1000μg/L(0.163±0.006,P<0.01)时,其对椎间盘细胞中Sox9基因mRNA可起到显著的正向调控作用;在此浓度下,它也可以对Ⅱ型胶原和蛋白聚糖基因mRNA起到正向调控作用。结论BMP2可以按照剂量依赖方式正向调控椎间盘细胞中Sox9、Ⅱ型胶原和蛋白聚糖基因的表达。Objective To assess the regulatory effects of bone morphogenetic protein-2 (BMP-2) on the gene expression of cartilage specificity gene Sox9,Type Ⅱ collagen and aggrecan mRNA in the human intervertebral discs.Methods RT-PCR was used to investigate the effects of BMP-2 on the gene expression of Sox9,Type Ⅱ collagen and aggrecan mRNA in intervertebral discs cells cultures of embryo.Results Exposure to 100 μg/L (0.149± 0.006, P< 0.05) and 1?000 μg/L (0.163± 0.006, P< 0.01) BMP-2 in serum-free medium caused a significant dose-dependent stimulation compared to 0 μg/L of Sox9 mRNA.On the same concentrations,BMP-2 could also increase the expression of mRNA of Type II collagen and aggrecan genes in a dose-dependent manner in the intervertebral discs.Conclusion BMP-2 could cause a dose-dependent stimulation on Sox9,Type Ⅱ collagen and aggrecan gene expression in human discs.

关 键 词：骨形态发生蛋白-2 椎间盘细胞软骨特异性基因 MRNA Ⅱ型胶原 椎间盘退变 

分 类 号：R681.5[医药卫生—骨科学]