一种脂肽类生物表面活性剂产生菌的筛选  

Isolation and Screening of Lipopeptide Biosurfactant Producing Bacterium

作　　者：吕应年[1] 杨世忠[1] 牟伯中[1] 

机构地区：[1]华东理工大学化学系

出　　处：《微生物学杂志》2005年第2期4-8,共5页Journal of Microbiology

基　　金：国家自然科学基金(59974014);教育部博士点基金(20030251002);教育部重点项目(03071);上海市教委资助项目

摘　　要：从油田地层水中筛选分离得到1株能够产生表面活性剂的细菌,经鉴定为枯草芽孢杆菌。分析了该菌株的生理形态和生长特性,以及该菌株代谢产生的生物表面活性剂的性质。薄层色谱与原位水解显色和红外光谱分析表明,培养后菌株代谢产生的生物表面活性为脂肽。它能使水的表面张力降低到26mN/m,其临界胶束浓度为0.025mg/mL。A biosurfactant producing bacterium was isolated and screened from aquifer stratum in an oil field and {identified} as Bacillus subtilis. Its physiological morphology, growth, and the biosurfactant properties of metabolite it produced were analyzed. Analysis by thin layer chromatography, original spot hydrolyzed-ninhydrin color, infrared spectrum indicated that the biosurfactant of metabolite after culturing of the strain was lipopeptide. It can reduce surface tension of water to 26 mN/m, and its critical micelle concentration (CMC) was 0.025 mg/mL.

关 键 词：枯草芽孢杆菌 筛选 生长特性 生物表面活性剂 脂肽 

分 类 号：Q939[生物学—微生物学]