特应性皮炎患者趋化因子及其受体的研究  

Detection of Serum Chemokines and Their Receptors on Peripheral Blood CD4^+ T Lymphocytes in Patients with Atopic Dermatitis

作　　者：钱起丰 林立航[2] 张明霞 刘启文[3] 

机构地区：[1]深圳市皮肤病防治研究所,性病防治研究中心,518020 [2]广东医学院皮肤性病科 [3]深圳市儿童医院皮肤科

出　　处：《中华皮肤科杂志》2005年第6期351-353,共3页Chinese Journal of Dermatology

基　　金：广东省医学科研基金资助项目(A2003713)

摘　　要：目的探讨几种重要的趋化因子及其受体的表达在特应性皮炎(AD)发病中的作用。方法采用酶联免疫吸附试验检测39例AD患者及正常人血清中γ干扰素诱导蛋白-10(IP-10)、基质细胞衍生因子-1α(SDF-1α)、嗜酸粒细胞趋化因子、胸腺和活化调节的趋化因子(TARC)及巨噬细胞来源的趋化因子(MDC)等水平;同时用流式细胞仪分析外周血CD4+T细胞表面趋化因子受体CXCR3、CXCR4、CCR3、CCR4及CCR5的表达。结果与正常人对照组相比,AD患者血清SDF-1α、TARC和MDC水平显著升高(P<0.001),IP-10及嗜酸粒细胞趋化因子水平则无明显改变(P>0.05),外周血CXCR3、CCR3、CCR4及CCR5在CD4+T细胞表达水平显著增加(P<0.001);血清TARC和MDC水平的变化与疾病严重程度相关(r分别为0.669及0.409,P分别为<0.001及<0.01)。结论具有生物活性趋化因子及其受体介导的T细胞和嗜酸/嗜碱粒细胞的聚集、激活后释放的炎性介质在AD发病中起着重要作用。Objective To study the role of serum chemokines and their receptors in the pathogenesis of atopic dermatitis(AD).Methods Serum levels of interferon γ-inducible protein-10(IP-10),stromal cell-derived factor-1α (SDF-1α),eotaxin,thymus and activation-regulated chemokine(TARC) as well as macrophage-derived chemokine (MDC) were detected by enzyme-linked immunosor bent assay(ELISA) in 39 patients with AD and 39 normal controls.Meanwhile,chem okine receptors CXCR3,CXCR4,CCR3,CCR4 and CCR5 on peripheral blood CD4+T-lymp hocytes were analyzed by flow cytometry with a two-color immunofluorescent sta ining in 39 patients with AD.Results Serum levels of SDF-1α,TARC and MDC we re significantly higher(P<0.001 ) in patients with AD[(1 361.5 ± 352.5) pg /mL,(807.1 ± 606.7) pg/mL and (1 290.4 ± 494.6) pg/mL,respectively] than those i n the normal controls[(834.9 ± 223.6) pg/mL,(308.5 ± 183.0) pg/mL and (811.8 ± 196.6) pg/mL,respectively].There was no significant difference in serum leve ls of IP-10 and eotaxin (P > 0.05) between the patients[(123.6 ± 110.4) pg/mL a nd (68.7 ± 26.2) pg/mL,respectively]and controls [(100.7 ± 73.7) pg/mL and(66.8 ± 20.5) pg/mL,respectively].The expression of CXCR3,CCR3,CCR4 and CCR5 o n CD4+T cells was increased (P<0.001) in peripheral blood of the patients (87.2 % ± 10.2%,15.2% ± 13.8%,55.9% ± 9.9% and 7.4% ± 4.5%,respectively) in comp arison with those of the healthy controls (65.4% ± 10.2%,6.1% ± 5.5%,47.2% ± 8.2% and 2.3% ± 0.4 %,respectively).Serum TARC and MDC levels were associate d with the disease activity(r = 0.669 and 0.409,respectively;P<0.001 and P<0.01,respectively).Conclusions Both chemokines with biological activity a nd t heir receptors may lead to the recruitment and activation of T lymphocytes,eosi nophils and basophils,which appear to play an important role in the pathogenesi s of AD by releasing mediators of inflammation.

关 键 词：特应性皮炎 嗜酸粒细胞趋化因子 基质细胞衍生因子-1 酶联免疫吸附试验 受体CXCR3 流式细胞仪分析 疾病严重程度 AD患者 CCR3 CCR5 CXCR4 T细胞表达 CD4^+ 嗜碱粒细胞 诱导蛋白 γ干扰素 细胞表面 细胞来源 受体介导 生物活性 

分 类 号：R758.2[医药卫生—皮肤病学与性病学] R562.25[医药卫生—临床医学]