天然抗角蛋白单克隆抗体3B4的多反应性鉴定及其分子机制的初步探讨  

Identification of the Polyreactivity of Monoclonal Natural Anti-ker atin Autoantibody 3B4 and Analysis of Its Molecular Mechanism

作　　者：管海宏[1] 付萌[1] 夏汝山[1] 王刚[1] 刘玉峰[1] 

机构地区：[1]第四军医大学西京医院皮肤科,西安710032

出　　处：《中华皮肤科杂志》2005年第6期377-379,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金资助项目(30330510);军队重点基金资助项目(01Z086)

摘　　要：目的对天然抗角蛋白单克隆抗体3B4的多反应性进行鉴定,并探讨其分子基础。方法用间接酶联免疫吸附实验(ELISA)和免疫组化法检测3B4与多种抗原的反应活性,分析其可变区基因与氨基酸序列。结果3B4除与角蛋白和皮肤发生较强的反应外,尚能选择性与多种具有不同表位的抗原和不同的组织反应;同源性分析表明,其重、轻链可变区基因分别与胚系基因VH1J558.42及VK1am4高度同源;HCDR3区为该抗体区别于其他同源性较高的免疫球蛋白的惟一基序,主要由短的侧链氨基酸组成。结论天然抗角蛋白单克隆抗体3B4具有多反应性,由少量突变的胚系基因编码,HCDR3区的柔韧性可能是多反应性的分子基础之一。Objective To identify the polyreactivity of a monoclonal natural anti-keratin autoantibody 3B4 and to analyze its possible molecular me chanism.Methods enzyme-linked immunosorbent assay (ELISA)and immunohistoche mistry were applied to test the binding reactivity of 3B4 against different anti gens and tissues.The variable region genes and their amino acid composition wer e sequenced.Results 3B4 could reacted with a range of antigens and tissues,i n addition to keratin and skin.The variable region genes of its light chain and heavy chain showed high homology with germline genes VK1 am4 and VH1 J558.42.H CDR3 region,which mainly composed of short side chain amino acids(from 294 to 324 nucleotides around the heavy chain),was the only motif that differs from ot her highly homologous immunoglobulin genes.Conclusions The monoclonal natural anti-keratin autoantibody 3B4,with its variable region genes highly homologo us to germline genes,is highly polyreactive.The flexibility of HCDR3 may contr ibute to the polyreactivity.

关 键 词：抗角蛋白单克隆抗体 多反应性 初步探讨 分子机制 天然 鉴定 间接酶联免疫吸附实验 免疫组化法检测 分子基础 氨基酸序列 可变区基因 同源性分析 轻链可变区 免疫球蛋白 氨基酸组成 反应活性 组织反应 基因编码 种抗原 

分 类 号：R392.1[医药卫生—免疫学]