外用维A酸引起小鼠皮肤反应的研究  

Skin Reactions Induced by Topical Retinoic Acid in Mice

作　　者：吴秋菊[1] 马鹏程[1] 陈志强[1] 魏峻[1] 

机构地区：[1]中国医学科学院,中国协和医科大学皮肤病研究所,南京210042

出　　处：《中华皮肤科杂志》2005年第6期380-382,共3页Chinese Journal of Dermatology

摘　　要：目的观察在动物实验中外用0.1%全反式维A酸霜及其基质引起的皮肤反应。方法小鼠背部每天分别外用0.1%全反式维A酸霜及其基质,连续使用30d,肉眼观察皮肤变化,并用生物学仪器进行红斑和皮屑检测。结果外用0.1%全反式维A酸霜,肉眼观察第3天左右出现红斑、脱屑反应,第6天左右达高峰,第12天左右消失,继续用药至第30天,未见反应再现;仪器测得红斑值在第1天时已明显升高,第6天时达最高峰,并持续高峰状态至第9天,第11天时降至基本正常,至第30天,均维持在正常范围,皮屑指数在用药第3天时明显升高,第9天时达高峰,然后逐渐下降,第27天时降至正常。外用基质部位与用药前比无差异。结论在动物实验中,外用0.1%全反式维A酸霜引起的皮肤反应在不停药的情况下,呈现“出现-高峰-消退”的一过性规律。维A酸皮肤反应与基质无关。Objective To investigate the skin reactions induced by to pical 0.1% tretinoin and its vehicle in mice.Methods 0.1% tretinoin and its v ehicle were applied separately on mice backs,once a day for 30 days.The skin r eactions,including erythema and scaling,were observed macroscopically and meas ured by biological instruments.Results Macroscopically the skin reactions of erythema and scaling induced by topical tretinoin occurred on day 3 after applic ation,reached its peak on day 6 and disappeared on day 12,and neither erythema nor abnormal scaling appeared in spite of continuing application until day 30.The erythema value measured was enhanced significantly on day 1 after applicatio n,reached its peak on day 6,maintained until day 9 and became normal on day 11.From then to day 30,it maintained at the normal level.The scaling value measu red enhanced significantly on day 3 after application,reached its peak on day 9 and became normal on day 27.Topical application of the vehicle induced neither skin reaction nor any change of erythema and scaling value.Conclusions Topic al application of 0.1% tretinoin induces skin reaction characterized by erythema and scaling.The reaction,transient but not related to the vehicle,experience s a process of 'occurrence-peak-disappearance'.

关 键 词：皮肤反应 外用 小鼠 维A酸霜 动物实验 肉眼观察 全反式 连续使用 皮肤变化 反应再现 正常范围 天时 高峰 基质 红斑 生物学 用药前 一过性 仪器 出现 升高 

分 类 号：R751[医药卫生—皮肤病学与性病学]