血清真胰岛素和胰岛素原检测在评价胰岛素抵抗、β细胞功能中的意义  

Significance of Evaluating Insulin Resistance and the Function of Pancreatic β Cell by True Insulin and Proinsulin

作　　者：田小平[1] 徐宁[1] 黄惠健[1] 黄海波[1] 庄惠琴[1] 郝志强[1] 何浩明[1] 

机构地区：[1]连云港市第一人民医院,江苏连云港222002

出　　处：《现代检验医学杂志》2005年第3期4-6,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨血清真胰岛素和胰岛素原在评价胰岛素抵抗和胰岛β细胞功能中的意义。方法71例新诊2型糖尿病(2DM)、63例糖耐量异常(IGT)和77例正常糖耐量(NGT)三组,根据其体重指数(BMI)分为肥胖组和非肥胖组。分别用酶联免疫吸附法(ELISA)测定空腹血清真胰岛素(trueinsulin,TI)、胰岛素原(proinsulin,PI)和用放射免疫法(RIA)测定的免疫反应性胰岛素(immunereactiveinsulin,IRI),比较用HOMA模型计算胰岛素抵抗(Homa-IR)和胰岛β细胞功能(Homa-β)值,探讨其间的意义。结果以TI计算的Homa-β值更能反应胰岛β细胞功能衰退的程度;随着糖耐量损害的加重,PI呈逐渐递增趋势,也是反应胰岛素抵抗、β细胞功能的敏感指标。结论以检测血清胰岛素和胰岛素原来量化分析和研究对象的胰岛素抵抗、胰岛β细胞功能在糖尿病的防治工作中有重要意义。真胰岛素在研究中的可信度高于免疫反应性胰岛素。Objective Analysis of the significance of evaluating insulin resistance (HOMA-IR) and the function of island β cell (HOMA-β) by true insulin(TI),immune reactive insulin(IRI) and proinsulin(PI).Methods TI,PI and IRI were measured in fasting sera of newly diagnosed patients with type 2 DM(n=71),subjects of IGT (n=63) and subjects with normal glucose tolerance (NGT,n=77),HOMA-IR and HOMA-β were calculated by HOMA model.Based on their body mass index (BMI) they were divided into obese subgroup and non-obese subgroup.Results Both the HOMA-IR and HOMA-β by using TI were significantly lower than that by using IRI (P<0.05);the increasing trend of PI concentration was appearing from NGT group to IGT group and to 2DM group.Conclusion There are very important significance of quantitative studying the HOMA-IR and HOMA-β of the subjects using TI and PI;TI is more reliable in the study than IRI.

关 键 词：真胰岛素 胰岛素原 胰岛素抵抗 胰岛Β细胞功能 

分 类 号：R587[医药卫生—内分泌] R446.112[医药卫生—内科学]