特发性男性不育DYS1基因微缺失研究  

The DYS1 gene microdeletion Of Idiopathic male Infertilities

作　　者：王卫国[1] 鹿占鹏[1] 杜敬民[1] 庞亚彤[1] 郭德荣[2] 

机构地区：[1]山东济宁市第一人民医院泌尿外科,济宁272100 [2]中山大学第五医院泌尿外科

出　　处：《中国男科学杂志》2005年第3期31-33,45,共4页Chinese Journal of Andrology

摘　　要：目的研究Y染色体长臂Yq11区DYS1基因微缺失与特发性男性不育的关系以及DYS1基因微缺失患者睾丸病理类型和性激素水平变化。方法病例组:60例临床诊断为特发性男性不育的患者;对照组:80例已生育的健康男性。外周血提取基因组DNA,以PCR的方法,检测目的基因。睾丸病理学检查采用组织活检、石蜡切片、HE染色、光镜下观察。血清性激素测定采用化学发光免疫分析法。结果病例组有8例患者出现DYS1基因微缺失,缺失率为13.33%(8/60),对照组无DYS1基因微缺失,两组DYS1基因的缺失率差异有显著统计学意义(P<0.01),可认为DYS1基因微缺失与特发性男性不育发病有关联(OR=26.07)。DYS1微缺失的患者睾丸病理均表现不同程度的生精细胞减少,其中3例生精细胞极少;DYS1微缺失的患者T水平低于对照组(P<0.01),也低于特发性男性不育组总体水平(P<0.05)。结论DYS1基因微缺失与特发性男性不育有关联,DYS1微缺失患者睾丸病理表现为不同程度的生精障碍,血清T水平低下。Objective To investigate the microdeletion of DYS1 gene in the long arm of Y chromosome of idiopathic male infertilities. And to investigate the testis pathology and the serum sexual hormone change of male infertilities with DYS1 gene microdeletion. Methods There were sixty idiopathic male infertilities in the experiment group and eighty healthy males in the control group. DYS1 gene was amplified by PCR method. The testis biopsies were embeded in paraffin, stained with H-E, observed under microscope. The serum sexual hormone was test by competitive immunassay method. Results There are eight patients with DYS1 gene microdeletion in the experiment group, none in the control group. The deletion ratio of DYS1 gene is 13.33%(8/60) in the experiment group. The ratio of microdeletion of DYS1 gene is statistic significant between the experiment group and the control group (P<0.01). The microdeletion of DYS1 gene has relationship with male infertility (OR=13.76). The damage of testis of DYS1 gene microdeletions was various. The mean of serum T of experiment group is lower than that of control group (P<0.01). Conclusion There is relationship between the microdeletion of DYS1 gene and male infertility.

关 键 词：特发性男性不育 DYS1基因 睾丸活检 性激素 

分 类 号：R698.2[医药卫生—泌尿科学]