输精管结扎对大鼠睾丸、附睾及血清睾酮的中长期影响  

The effects of long-term vasectomy on the serum testosterone, testis and epididymis tissues

作　　者：巩念明[1] 刘执玉[1] 毕玉顺[1] 胡坚利[1] 田广平[1] 宋涛[1] 

机构地区：[1]山东大学医学院淋巴学研究室,济南250012

出　　处：《中国临床解剖学杂志》2005年第3期284-286,共3页Chinese Journal of Clinical Anatomy

基　　金：山东省自然科学基金项目(Y2002-C01)

摘　　要：目的:建立动物模型,观测输精管结扎中、长期对血清睾酮水平及睾丸和附睾的形态学影响,为评估输精管结扎术这一男性节育主要手段提供某些基础理论依据。方法:随机将40只4月龄Wistar大鼠,按4个等长的实验间期分为结扎组和对照组,在术后第4、6、8、10月时分别测定结扎组和对照组的血清睾酮(ST)浓度及其与雄激素结合蛋白(ABP)的结合率;在对受试动物的睾丸和附睾进行组织学定性观察的同时,用TAS-plus型自动图像分析仪,对睾丸切片进行了定量组织学测定。结果:除第4月实验组和对照组外,其他3个间期的实验组和对照组血清睾酮浓度均有显著变化;实验组睾丸和附睾均有无菌性炎症发生,且未随术后时间的延长而缓解。结论:结扎术对受试动物的中长期影响值得关注。Objective: To study the long-term effects of vasectomy on serum testosterone, testis and epididymis tissues and evaluate the value of vasectomy for male fertility control. Methods: 40 sexually mature rats were divided into two groups: control group and surgery group. The later were taken vasectomy. After 4, 6, 8, 10 months of operation, serum testosterone (ST) levels and its binding rate with ABP were detected, and testis and epididymis of model rats were observed. Meanwhile, we investigated the testicular tissue quantitatively with a TAS-plus automated image analyzer. Results: There were significant difference of testosterone levels between surgery and control groups after 6, 8, 10 months. Aseprtic inflammation was found in the testis and epididymis of surgery groups. Conclusions: Long-term vasectomy can reduce serum testosterone levels and cause some histological change in testis and epididymis.

关 键 词：输精管结扎 血清睾酮 睾丸 附睾 动物模型 大鼠 

分 类 号：R322.64[医药卫生—人体解剖和组织胚胎学]