肾素-血管紧张素系统基因多态性与糖尿病及合并肾病的关系  

Associations of the polymorphisms in the renin-angiotensin system with type 2 diabetes mellitus and diabetic nephropathy

作　　者：钟卫干[1] 覃甲仁[2] 贺菽嘉[2] 

机构地区：[1]广西中医学院基础医学院生物化学教研室,广西南宁530001 [2]广西医科大学基础医学院生物化学与分子生物学教研室,广西南宁530021

出　　处：《基础医学与临床》2005年第5期446-450,共5页Basic and Clinical Medicine

摘　　要：目的探讨血管紧张素I转换酶(ACE)基因及血管紧张素原(AGT)基因与2型糖尿病(DM)及合并糖尿病肾病(DN)的相关性。方法分别用PCR、突变基因分离聚合酶链反应(MS-PCR)技术对195例2型DM患者和136例正常对照者的ACEI/D与AGTM235T多态性进行检测。结果(1)DM组ACE-DD基因型和D等位基因频率均比对照组显著增高(P<0.001)。(2)DN(+)组ACE基因型和等位基因频率与DN(-)组比较无显著性差异。(3)AGT基因型分布与等位基因频率在3组中均无显著性差异。(4)联合分析ACE-DD型及AGT-TT型对DN(+)、DN(-)的OR分别为4.17和3.16;均高于单基因DD型及TT型的OR值。结论(1)ACEDD基因型和D等位基因可能是广西地区人群2型DM的易感因素。(2)未发现ACEI/D或AGTM235T多态性单一因素与2型DM患者中DN的发生有关联。(3)ACE-DD基因型与AGTM235T-TT基因型在2型DM及DN发生中有协同作用。ObjectiveTo investigate whether two polymorphisms in the renin-angiotensin system(RAS)-ACE I/D and AGT M235T are associated with type 2 DM and DN. MethodsA case-control study was performed in 195 type 2 DM patients and 136 controls. The ACE I/D and AGT M235T polymorphisms were examed by PCR and mutagenically separated PCR(MS-PCR) respectively. [WT10.HZResults(1)The frequencies of ACE-DD genotype and ACE D allele were significantly higher in DM group than that in control group (P<0.001,respectively).(2)The ACE I/D polymorphism did not show any difference between DN(+) and DN(-) groups.(3) No significant differences in AGT M235T polymorphism among three groups. (4) DN(+) OR associated with a combined genotype(ACE-DD + AGT M235T-TT) was 4.17,DN(-) OR associated with this combined genotype was 3.16.Both were increased than those associated with single ACE-DD or AGT M235T-TT genotype. Conclusion(1) ACE-DD genotype and D allele may be risk factors of type 2 DM in Guangxi population. (2) None of the two polymorphisms (ACE I/D,AGT M235T) contributes to genetic susceptibility to DN in type 2 DM patients. ((3) The) results suggested a synergistic effect between ACE-DD genotype and AGT M235T-TT genotype on DN in type 2 DM.

关 键 词：肾素-血管紧张素系统 基因多态性 M235T多态性 血管紧张素Ⅰ转换酶 等位基因频率 DD基因型 糖尿病(DM) 显著性差异 2型DM 肾病(DN) 血管紧张素原 聚合酶链反应 基因型分布 D等位基因 ACE 正常对照 基因分离 E基因型 联合分析 

分 类 号：R587.2[医药卫生—内分泌]