调心方活性部位对β淀粉样蛋白抑制大鼠海马脑片CA1区诱发长时程增强的作用研究  

Inhibitory Effect of Active Fraction of Tiaoxin Recipe on β-Amyloid Protein Induced Long-term Potentiation in CA1 Area of Rats' Hippocampal Slices

作　　者：乔海法[1] 杨胜[1] 周文霞[1] 张永祥[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国中西医结合杂志》2005年第5期429-431,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金重点项目(No.39830450)

摘　　要：目的研究调心方活性部位A(activefractionofTiaoxinrecipe,TXR A)对β-淀粉样蛋白(betaarnyloidprotein ,βAP)抑制大鼠海马脑片长时程增强(long term potentiation ,LTP)效应的影响。方法采用细胞外微电极记录技术,记录大鼠海马脑片CA1区诱发群峰电位(populationspike,PS) ,然后施以10 0Hz,10 0串的强直刺激诱发LTP。结果与正常脑脊液组比较,β- AP(0. 2 μmol/L)孵育海马脑片>1 5h ,强直刺激后PS增幅程度显著降低,提示β- AP对海马LTP具有抑制作用;如用β- AP加TXR- A孵育脑片>1 5h ,则强直刺激后高浓度TXR -A组的PS平均幅度显著提高,且作用优于调心方,提示TXR- A可以增强LTP幅度。结论TXR A可能是发挥调心方改善β- AP抑制大鼠海马CA1区诱发LTP作用的主要成分之一,拮抗β- AP对LTP的抑制可能是其益智机制之一。ObjectiveTo study the effect of the active fraction of Tiaoxin recipe (TXR-A), in inhibiting long-term potentiation (LTP) induced by beta amyloid protein (β-AP) in CA1 area of rats' hippocampal slices. MethodsThe population spike (PS) in CA1 area of hippocampal slices incubated in different medium was recorded before and after LTP was evoked by a 100Hz, 100 trains high frequency stimulation (HFS), using extracellular microelectrode recording techniques. ResultsThe amplitude of PS significantly decreased after HFS in hippocampal slices incubated in medium containing 0.2 μmol/L β-AP for more than 1.5 hour, as compared with that incubated in normal cerebrospinal fluid, the difference was significant, suggesting that β-AP could inhibit LTP in hippocampal slices. The average amplitude of PS in slices incubated in β-AP containing medium could be significantly enhanced by adding high-concentration TXR-A or TXR into the medium, and TXR-A showed a better effect of enhancing than that of TXR, indicating that TXR-A could increase the amplitude of LTP. ConclusionTXR-A may be the chief ingredient extracted from TXR for improving β-AP induced LTP in CA1 area of rats' hippocampus, to antagonise the inhibition of β-AP on LTP is possibly one of the mechanisms for its intelligence benefiting action.

关 键 词：长时程增强 海马脑片 活性部位 调心方 诱发 大鼠 作用研究 蛋白抑制 Β淀粉样 β-淀粉样蛋白 protein 微电极记录技术 β-AP 强直刺激 海马CA1区 100Hz 正常脑脊液 海马LTP 群峰电位 抑制作用 主要成分 细胞外 孵育 PS 

分 类 号：R285[医药卫生—中药学]