安宫牛黄散中的朱砂雄黄对感染性脑水肿大鼠乳酸脱氢酶及其同工酶的影响  

Effects of Cinnabar and Realgar in Angong Niuhuang Powder on Lactate Dehydrogenase and Its Isoenzymes in Rats with Infectious Cerebral Edema

作　　者：汤毅珊[1] 林璞粤[1] 欧卫平[1] 王宁生[1] 

机构地区：[1]广州中医药大学临床药理研究所,广州510405

出　　处：《中国中西医结合杂志》2005年第5期436-440,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：广东省自然科学基金资助项目(No.010270)

摘　　要：目的研究生理、病理状态下安宫牛黄散中的朱砂、雄黄对机体作用的不同特点,探讨复方中朱砂、雄黄的药理作用机制。方法将SD大鼠随机分成6组(8～10只/组) :正常组,正常加安宫牛黄散(下简称整方)组(2 78mg/kg) ;正常加除去朱砂、雄黄的安宫牛黄散(下简称拆方)组(2 2 2. 7mg/kg) ;脑水肿模型组(一侧大鼠颈总动脉注射百日咳杆菌2 50亿/kg) ;模型加整方组(造模前1h给药2 78mg/kg) ;模型加拆方组(造模前1h给药2 2 2 . 7mg/kg)。一次给药后5h(模型组注菌后4h)采血、制备脑匀浆。用比色法测定脑组织、血清中乳酸脱氢酶(LDH)总活力,琼脂糖凝胶电泳法测定血清中乳酸脱氢酶同工酶LDH1 -5 百分酶活力。结果与正常组比较,模型组LDH总活力、LDH1 、LDH2 百分酶活力显著升高(P <0 .0 1) ;正常加整方组、正常加拆方组LDH总活力升高(P <0 . 0 5) ,LDH1 、LDH2 百分酶活力均显著升高(P <0 . 0 1)。LDH4、LDH5 百分酶活力下降(P <0. 0 1) ,但除LDH5 外,整方组和拆方组各项酶活力组间差异无显著性。与模型组比较,模型加整方组、模型加拆方组LDH总活力显著下降(P <0 . 0 1) ,LDH5 百分酶活力显著升高(P <0 .0 1) ,但两组间差异无显著性;模型加整方组LDH2 、LDH3 百分酶活力显著下降(P <0 . 0 1) ,LDH1 。ObjectiveTo explore the pharmacologic mechanism of cinnabar (CA) and realgar (RG) in Angong Niuhuang powder (ANP) by way of studying the characteristics of their effects on organism under physiologic and pathologic states. MethodsSD rats were randomly divided into six groups, 8-10 rats in each group. Group A: untreated normal rats; Group B: normal rats administered by ANP (drug I) 278 mg/kg; Group C: normal rats administered by ANP subtracted CA and RG (drug Ⅱ) 222.7 mg/kg; Group D: brain edema model rats established by unilateral common carotid artery injection of Bacillus pertussis 250 million/kg; Group E: model rats administered by ANP 278 mg/kg 1 hr before modeling; Group F: model rats administered by drug Ⅱ 222.7 mg 1 hr before modeling. Blood sample and brain tissue in Group D were obtained 4 hrs after modeling and those in other groups obtained 5 hrs after drug administration. The total activity of lactate dehydrogenase (LDH) in serum and brain tissue was determined by colorimetry and that of serum LDH isoenzymes (LDH_ 1-5 ) were determined by gel electrophoresis. ResultsAs compared with Group A, LDH, LDH_1 and LDH_2 activities increased in Group D (P<0.01), and increased also in Group B and C (P<0.05), while LDH_4 and LDH_5 decreased obviously in Group B and C. But except that of LDH_5, no significant difference of LDH_ 1-4 in brain tissue and serum was shown in comparison of Group B and C. As compared with Group D, LDH was lower (P<0.01) and LDH_5 was higher (P<0.01) in Group E and F without significant difference, LDH_2,LDH_3 were lower in Group E (P<0.01) but unchanged in Group F, LDH_1 and LDH_4 were not changed in Group E but significantly lowered in Group F (P<0.05 and P<0.01). ConclusionAdministration of ANP in normal physiologic condition would cause damage on myocardium and kidney to certain extent, administration of ANP and drug Ⅱ in pathologic (infectious brain edema) would suppress the hyper-activated LDH, with no significant difference between the effects of drug Ⅱ and ANP. Howev

关 键 词：安宫牛黄散 感染性脑水肿 雄黄 朱砂 乳酸脱氢酶同工酶 琼脂糖凝胶电泳法 LDH1 药理作用机制 颈总动脉注射 酶活力 病理状态 百日咳杆菌 比色法测定 显著性 SD大鼠 一次给药 0.05 活力变化 生理状态 损伤作用 过度激活 

分 类 号：R285[医药卫生—中药学]